|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online March 23, 2006
doi: 10.1242/10.1242/dev.02322
1 Skaggs School of Pharmacy, University of California, San Diego, 9500 Gilman
Drive, La Jolla, CA 92093, USA.
2 Department of Medicine, University of California, San Diego, 9500 Gilman
Drive, La Jolla, CA 92093, USA.
3 Cardiovascular Research Center, Massachusetts General Hospital, Harvard
Medical School, Boston, 185 Cambridge Street, MA 02114, USA.
4 Hubrecht Laboratory (Netherlands Institute for Developmental Biology),
Utrecht, The Netherlands.
5 Interuniversity Cardiology Institute of the Netherlands, Utrecht, The
Netherlands.
6 Leon H. Charney Division of Cardiology, New York University School of
Medicine, New York, NY 10016, USA.
* Author for correspondence (e-mail: syevans{at}ucsd.edu)
Accepted 13 February 2006
A number of human congenital disorders present with both heart and limb defects, consistent with common genetic pathways. We have recently shown that the LIM homeodomain transcription factor islet 1 (Isl1) marks a subset of cardiac progenitors. Here, we perform lineage studies with an Isl1Cre mouse line to demonstrate that Isl1 also marks a subset of limb progenitors. In both cardiac and limb progenitors, Isl1 expression is downregulated as progenitors migrate in to form either heart or limb. To investigate common heart-limb pathways in Isl1-expressing progenitors, we ablated the Type I Bmp receptor, Bmpr1a utilizing Isl1Cre/+. Analysis of consequent heart and limb phenotypes has revealed novel requirements for Bmp signaling. Additionally, we find that Bmp signaling in Isl1-expressing progenitors is required for expression of T-box transcription factors Tbx2 and Tbx3 in heart and limb. Tbx3 is required for heart and limb formation, and is mutated in ulnar-mammary syndrome. We provide evidence that the Tbx3 promoter is directly regulated by Bmp Smads in vivo.
Key words: Isl1, Bmp, Tbx2, Tbx3, Heart, Hindlimb
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  K. D. Boudoulas and A. K. Hatzopoulos Cardiac repair and regeneration: the Rubik's cube of cell therapy for heart disease Dis. Model. Mech., July 1, 2009; 2(7-8): 344 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Harvey, S. M. Meilhac, and M. E. Buckingham Landmarks and Lineages in the Developing Heart Circ. Res., June 5, 2009; 104(11): 1235 - 1237. [Full Text] [PDF]
|
|
|
|
|
|
F. Rochais, K. Mesbah, and R. G. Kelly Signaling Pathways Controlling Second Heart Field Development Circ. Res., April 24, 2009; 104(8): 933 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Chien, I. J. Domian, and K. K. Parker Cardiogenesis and the Complex Biology of Regenerative Cardiovascular Medicine Science, December 5, 2008; 322(5907): 1494 - 1497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. El-Bizri, C. Guignabert, L. Wang, A. Cheng, K. Stankunas, C.-P. Chang, Y. Mishina, and M. Rabinovitch SM22{alpha}-targeted deletion of bone morphogenetic protein receptor 1A in mice impairs cardiac and vascular development, and influences organogenesis Development, September 1, 2008; 135(17): 2981 - 2991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nathan, A. Monovich, L. Tirosh-Finkel, Z. Harrelson, T. Rousso, A. Rinon, I. Harel, S. M. Evans, and E. Tzahor The contribution of Islet1-expressing splanchnic mesoderm cells to distinct branchiomeric muscles reveals significant heterogeneity in head muscle development Development, February 15, 2008; 135(4): 647 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Hardy, L. Li, Z. Wang, J. Sedy, J. Fawcett, E. Frank, J. Kucera, and T. Pawson Combinatorial ShcA Docking Interactions Support Diversity in Tissue Morphogenesis Science, July 13, 2007; 317(5835): 251 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lin, L. Cui, W. Zhou, D. Dufort, X. Zhang, C.-L. Cai, L. Bu, L. Yang, J. Martin, R. Kemler, et al. beta-Catenin directly regulates Islet1 expression in cardiovascular progenitors and is required for multiple aspects of cardiogenesis PNAS, May 29, 2007; 104(22): 9313 - 9318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Goddeeris, R. Schwartz, J. Klingensmith, and E. N. Meyers Independent requirements for Hedgehog signaling by both the anterior heart field and neural crest cells for outflow tract development Development, April 15, 2007; 134(8): 1593 - 1604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-A. Renard, C. Labalette, C. Armengol, D. Cougot, Y. Wei, S. Cairo, P. Pineau, C. Neuveut, A. de Reynies, A. Dejean, et al. Tbx3 Is a Downstream Target of the Wnt/{beta}-Catenin Pathway and a Critical Mediator of {beta}-Catenin Survival Functions in Liver Cancer Cancer Res., February 1, 2007; 67(3): 901 - 910. [Abstract] [Full Text] [PDF]
|
|
