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First published online 13 June 2007
doi: 10.1242/dev.000877

Development 134, 2615-2625 (2007)
Published by The Company of Biologists 2007
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Defective osteoblast function in ICAP-1-deficient mice

Daniel Bouvard1,2,3,*, Attila Aszodi3, Günter Kostka3, Marc R. Block1,2, Corinne Albigès-Rizo1,2 and Reinhard Fässler3

1 Université Joseph Fourier, CNRS, UMR 5538, LEDAC, Institut Albert Bonniot, La Tronche Cedex, F-38706, France.
2 INSERM, U823, Equipe DySAD, Institut Albert Bonniot, F-38042, France.
3 Max Planck Institut für Biochemie, Department of Molecular Medicine, Am Klopferspitz 18a, 82152 Martinsried, Germany.

* Author for correspondence (e-mail: daniel.bouvard{at}ujf-grenoble.fr)

Accepted 4 May 2007

The integrin receptor family plays important roles in cell-to-cell and cell-to-extracellular matrix interactions through the recruitment of accessory molecules. One of them, the integrin cytoplasmic domain-associated protein-1 (ICAP-1; also known as ITGB1BP1), specifically interacts with the cytoplasmic domain of the ß1 integrin subunit and negatively regulates its function in vitro. To address the role of ICAP-1 in vivo, we ablated the Icap-1 gene in mice. We report an unexpected role of ICAP-1 in osteoblast function during bone development. Icap-1-deficient mice suffer from reduced osteoblast proliferation and delayed bone mineralization, resulting in the retarded formation of bone sutures. In vitro studies reveal that primary and immortalized Icap-1-null osteoblasts display enhanced adhesion and spreading on extracellular matrix substrates, probably owing to an increase in ß1 integrin activation. Finally, we provide evidence that ICAP-1 promotes differentiation of osteoprogenitors by supporting their condensation through modulating the integrin high affinity state.

Key words: ICAP-1 (ITGB1BP1), Integrin, Cell differentiation, Cell adhesion, Osteoblast, Mouse




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
A. Millon-Fremillon, D. Bouvard, A. Grichine, S. Manet-Dupe, M. R. Block, and C. Albiges-Rizo
Cell adaptive response to extracellular matrix density is controlled by ICAP-1-dependent {beta}1-integrin affinity
J. Cell Biol., January 28, 2008; 180(2): 427 - 441.
[Abstract] [Full Text] [PDF]


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