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First published online 1 August 2007
doi: 10.1242/dev.001214

Development 134, 3145-3153 (2007)
Published by The Company of Biologists 2007
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A role for the Myoblast city homologues Dock1 and Dock5 and the adaptor proteins Crk and Crk-like in zebrafish myoblast fusion

Catherine A. Moore, Caroline A. Parkin*, Yannick Bidet{dagger} and Philip W. Ingham*,{ddagger}

MRC Centre for Developmental and Biomedical Genetics, Department of Biomedical Science, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK.

{ddagger} Author for correspondence (e-mail: p.w.ingham{at}sheffield.ac.uk)

Accepted 25 June 2007

Myoblast fusion follows a defined sequence of events that is strikingly similar in vertebrates and invertebrates. Genetic analysis in Drosophila has identified many of the molecules that mediate the different steps in the fusion process; by contrast, the molecular basis of myoblast fusion during vertebrate embryogenesis remains poorly characterised. A key component of the intracellular fusion pathway in Drosophila is the protein encoded by the myoblast city (mbc) gene, a close homologue of the vertebrate protein dedicator of cytokinesis 1 (DOCK1, formerly DOCK180). Using morpholino antisense-oligonucleotide-mediated knockdown of gene activity in the zebrafish embryo, we show that the fusion of embryonic fast-twitch myoblasts requires the activities of Dock1 and the closely related Dock5 protein. In addition, we show that the adaptor proteins Crk and Crk-like (Crkl), with which Dock proteins are known to interact physically, are also required for myoblast fusion.

Key words: Crk, Crkl, DOCK1, DOCK5, Myoblast city, Myoblast fusion, Zebrafish


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