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First published online September 7, 2007
doi: 10.1242/10.1242/dev.010298

Development 134, 3539-3548 (2007)
Published by The Company of Biologists 2007
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Pattern formation during de novo assembly of the Arabidopsis shoot meristem

Sean P. Gordon1, Marcus G. Heisler1, G. Venugopala Reddy1,2, Carolyn Ohno1, Pradeep Das1,3 and Elliot M. Meyerowitz1,*

1 Division of Biology, California Institute of Technology, Pasadena, CA, USA.
2 Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
3 Laboratorie RDP, Ecole Normale Superieur de Lyon, Lyon, France.

* Author for correspondence (e-mail: meyerow{at}caltech.edu)

Accepted 21 July 2007

Most multicellular organisms have a capacity to regenerate tissue after wounding. Few, however, have the ability to regenerate an entire new body from adult tissue. Induction of new shoot meristems from cultured root explants is a widely used, but poorly understood, process in which apical plant tissues are regenerated from adult somatic tissue through the de novo formation of shoot meristems. We characterize early patterning during de novo development of the Arabidopsis shoot meristem using fluorescent reporters of known gene and protein activities required for shoot meristem development and maintenance. We find that a small number of progenitor cells initiate development of new shoot meristems through stereotypical stages of reporter expression and activity of CUP-SHAPED COTYLEDON 2 (CUC2), WUSCHEL (WUS), PIN-FORMED 1 (PIN1), SHOOT-MERISTEMLESS (STM), FILAMENTOUS FLOWER (FIL, also known as AFO), REVOLUTA (REV), ARABIDOPSIS THALIANA MERISTEM L1 LAYER (ATML1) and CLAVATA 3 (CLV3). Furthermore, we demonstrate a functional requirement for WUS activity during de novo shoot meristem initiation. We propose that de novo shoot meristem induction is an easily accessible system for the study of patterning and self-organization in the well-studied model organism Arabidopsis.

Key words: Auxin, Callus, Cytokinin, Regeneration, Self-organization, Shoot meristem, Arabidopsis thaliana


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