spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online September 7, 2007
doi: 10.1242/10.1242/dev.006569

Development 134, 3549-3563 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lavial, F.
Right arrow Articles by Pain, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lavial, F.
Right arrow Articles by Pain, B.

The Oct4 homologue PouV and Nanog regulate pluripotency in chicken embryonic stem cells

Fabrice Lavial1, Hervé Acloque1,*, Federica Bertocchini2, David J. MacLeod3, Sharon Boast2, Elodie Bachelard1, Guillaume Montillet1, Sandrine Thenot1, Helen M. Sang3, Claudio D. Stern2, Jacques Samarut1 and Bertrand Pain4,5,{dagger}

1 Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Université Lyon 1, CNRS, INRA, Ecole Normale Supérieure de Lyon, France.
2 Department of Anatomy and Developmental Biology, University College London, London, UK.
3 Roslin Institute, Roslin, Midlothian EH25 9PS, UK.
4 Inserm, U384, Faculté de Médecine, 28, place Henri Dunant, Clermont-Ferrand, F-63001, France.
5 Département de Physiologie Animale, INRA, France.

{dagger} Author for correspondence (e-mail: bepain{at}u-clermont1.fr)

Accepted 16 July 2007

Embryonic stem cells (ESC) have been isolated from pregastrulation mammalian embryos. The maintenance of their pluripotency and ability to self-renew has been shown to be governed by the transcription factors Oct4 (Pou5f1) and Nanog. Oct4 appears to control cell-fate decisions of ESC in vitro and the choice between embryonic and trophectoderm cell fates in vivo. In non-mammalian vertebrates, the existence and functions of these factors are still under debate, although the identification of the zebrafish pou2 (spg; pou5f1) and Xenopus Pou91 (XlPou91) genes, which have important roles in maintaining uncommitted putative stem cell populations during early development, has suggested that these factors have common functions in all vertebrates. Using chicken ESC (cESC), which display similar properties of pluripotency and long-term self-renewal to mammalian ESC, we demonstrated the existence of an avian homologue of Oct4 that we call chicken PouV (cPouV). We established that cPouV and the chicken Nanog gene are required for the maintenance of pluripotency and self-renewal of cESC. These findings show that the mechanisms by which Oct4 and Nanog regulate pluripotency and self-renewal are not exclusive to mammals.

Key words: Nanog, Oct4, Avian homologue, cPouV, Stem cells




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Rodriguez-Leon, C. R. Esteban, M. Marti, B. Santiago-Josefat, I. Dubova, X. Rubiralta, and J. C. I. Belmonte
Pitx2 regulates gonad morphogenesis
PNAS, August 12, 2008; 105(32): 11242 - 11247.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007