spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 12 September 2007
doi: 10.1242/dev.009522

Development 134, 3585-3592 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.009522v1
134/20/3585    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walsh, C. M.
Right arrow Articles by Carroll, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walsh, C. M.
Right arrow Articles by Carroll, S. B.

Collaboration between Smads and a Hox protein in target gene repression

Christopher M. Walsh and Sean B. Carroll*

Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA.

* Author for correspondence (e-mail: sbcarrol{at}wisc.edu)

Accepted 7 August 2007

Hox proteins control the differentiation of serially iterated structures in arthropods and chordates by differentially regulating many target genes. It is yet unclear to what extent Hox target gene selection is dependent upon other regulatory factors and how these interactions might affect target gene activation or repression. We find that two Smad proteins, effectors of the Drosophila Dpp/TGF-ß pathway, that are genetically required for the activation of the spalt (sal) gene in the wing, collaborate with the Hox protein Ultrabithorax (Ubx) to directly repress sal in the haltere. The repression of sal is integrated by a cis-regulatory element (CRE) through a remarkably conserved set of Smad binding sites flanked by Ubx binding sites. If the Ubx binding sites are relocated at a distance from the Smad binding sites, the proteins no longer collaborate to repress gene expression. These results support an emerging view of Hox proteins acting in collaboration with a much more diverse set of transcription factors than has generally been appreciated.

Key words: Hox proteins, Repression, Smad proteins, Collaboration, Combinatorial regulation


Related articles in Development:

Hox function through collaboration

Development 2007 134: e2001. [Full Text]  



This article has been cited by other articles:


Home page
 DevelopmentHome page
T. Hayashi, C. Xu, and R. W. Carthew
Cell-type-specific transcription of prospero is controlled by combinatorial signaling in the Drosophila eye
Development, August 15, 2008; 135(16): 2787 - 2796.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Liu, K. S. Matthews, and S. E. Bondos
Multiple Intrinsically Disordered Sequences Alter DNA Binding by the Homeodomain of the Drosophila Hox Protein Ultrabithorax
J. Biol. Chem., July 25, 2008; 283(30): 20874 - 20887.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. M. Walsh and S. B. Carroll
Collaboration between Smads and a Hox protein in target gene repression
J. Cell Sci., October 15, 2007; 120(20): e2006 - e2006.
[Full Text]


© The Company of Biologists Ltd 2007