spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online October 12, 2007
doi: 10.1242/10.1242/dev.008276

Development 134, 3905-3915 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kagoshima, H.
Right arrow Articles by Woollard, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kagoshima, H.
Right arrow Articles by Woollard, A.

The C. elegans CBFß homologue BRO-1 interacts with the Runx factor, RNT-1, to promote stem cell proliferation and self-renewal

Hiroshi Kagoshima1,*,{dagger}, Rachael Nimmo2,*, Nicole Saad2, Junko Tanaka3, Yoshihiro Miwa3,4, Shohei Mitani5, Yuji Kohara1 and Alison Woollard2,{dagger}

1 Genome Biology Laboratory, National Institute of Genetics, Mishima 411-8560, Japan.
2 Genetics Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
3 Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba 305-8577, Japan.
4 Precursory Research and Embryonic Science and Technology (PRESTO), JST, Okazaki 444-8585, Japan.
5 Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.

{dagger} Author for correspondence (e-mail: hkagoshi{at}lab.nig.ac.jp; alison.woollard{at}bioch.ox.ac.uk)

Accepted 20 August 2007

In this report, we investigate the C. elegans CBFß homologue, BRO-1. bro-1 mutants have a similar male-specific sensory ray loss phenotype to rnt-1 (the C. elegans homologue of the mammalian CBFß-interacting Runx factors), caused by failed cell divisions in the seam lineages. Our studies indicate that BRO-1 and RNT-1 form a cell proliferation-promoting complex, and that BRO-1 increases both the affinity and specificity of RNT-1-DNA interactions. Overexpression of bro-1, like rnt-1, leads to an expansion of seam cell number and co-overexpression of bro-1 and rnt-1 results in massive seam cell hyperplasia. Finally, we find that BRO-1 appears to act independently of RNT-1 in certain situations. These studies provide new insights into the function and regulation of this important cancer-associated DNA-binding complex in stem cells and support the view that Runx/CBFß factors have oncogenic potential.

Key words: C. elegans, CBFß, Stem cell, Proliferation, Self-renewal, Runx


Related articles in Development:

Runx oncogenic potential wormed out

Development 2007 134: e2106. [Full Text]  





© The Company of Biologists Ltd 2007