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First published online October 26, 2007
doi: 10.1242/10.1242/dev.010272

Development 134, 4095-4106 (2007)
Published by The Company of Biologists 2007
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Lrp6 is required for convergent extension during Xenopus gastrulation

Emilios Tahinci1, Curtis A. Thorne1, Jeffrey L. Franklin1,2, Adrian Salic3, Kelly M. Christian1, Laura A. Lee1, Robert J. Coffey1,2 and Ethan Lee1,*

1 Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
2 Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
3 Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

* Author for correspondence (e-mail: ethan.lee{at}vanderbilt.edu)

Accepted 5 September 2007

Wnt signaling regulates ß-catenin-mediated gene transcription and planar cell polarity (PCP). The Wnt co-receptor, Lrp6, is required for signaling along the ß-catenin arm. We show that Lrp6 downregulation (by morpholino injection) or overexpression in Xenopus embryos disrupts convergent extension, a hallmark feature of Wnt/PCP components. In embryos with decreased Lrp6 levels, cells of the dorsal marginal zone (DMZ), which undergoes extensive cellular rearrangements during gastrulation, exhibit decreased length:width ratios, decreased migration, and increased numbers of transient cytoplasmic protrusions. We show that Lrp6 opposes Wnt11 activity and localizes to the posterior edge of migrating DMZ cells and that Lrp6 downregulation enhances cortical and nuclear localization of Dsh and phospho-JNK, respectively. Taken together, these data suggest that Lrp6 inhibits Wnt/PCP signaling. Finally, we identify the region of the Lrp6 protein with Wnt/PCP activity to a stretch of 36 amino acids, distinct from regions required for Wnt/ß-catenin signaling. We propose a model in which Lrp6 plays a critical role in the switch from Wnt/PCP to Wnt/ß-catenin signaling.

Key words: Lrp6, Wnt, Planar cell polarity, Convergent extension, Gastrulation, Xenopus




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