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First published online November 26, 2007
doi: 10.1242/10.1242/dev.010983

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Spermatocyte cytokinesis requires rapid membrane addition mediated by ARF6 on central spindle recycling endosomes

Naomi Dyer^1,2,*, Elena Rebollo^3,*, Paloma Domínguez³, Nadia Elkhatib⁴, Philippe Chavrier⁴, Laurent Daviet⁵, Cayetano González³ and Marcos González-Gaitán^1,6,

¹ Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
² Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK.
³ Institut de Recerca Biomèdica (IRB) and Institució Catalana de Recerca i Estudis Avançats (ICREA), Parc Cientific Barcelona, Josep Samitier 1-5, 08028 Barcelona, Spain.
⁴ CNRS UMR144, Institut Curie, Membrane and Cytoskeleton Dynamics Group, 26 Rue d'Ulm 75005 Paris, France.
⁵ Hybrigenics SA, 3-5 Impasse Reille, 75014 Paris, France.
⁶ Département de biochimie, Sciences II, 30, Quai Ernest Ansermet CH-1211 Genève 4, Switzerland.

Author for correspondence (e-mail: marcos.gonzalez{at}biochem.unige.ch)

Accepted 20 September 2007

The dramatic cell shape changes during cytokinesis require the interplay between microtubules and the actomyosin contractile ring, and addition of membrane to the plasma membrane. Numerous membrane-trafficking components localize to the central spindle during cytokinesis, but it is still unclear how this machinery is targeted there and how membrane trafficking is coordinated with cleavage furrow ingression. Here we use an arf6 null mutant to show that the endosomal GTPase ARF6 is required for cytokinesis in Drosophila spermatocytes. ARF6 is enriched on recycling endosomes at the central spindle, but it is required neither for central spindle nor actomyosin contractile ring assembly, nor for targeting of recycling endosomes to the central spindle. However, in arf6 mutants the cleavage furrow regresses because of a failure in rapid membrane addition to the plasma membrane. We propose that ARF6 promotes rapid recycling of endosomal membrane stores during cytokinesis, which is critical for rapid cleavage furrow ingression.

Key words: Drosophila, Meiosis, Spermatogenesis, Testis

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Membrane recycling: not an ARFterthought

Development 2007 134: e2402. [Full Text]  

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