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First published online 21 December 2006
doi: 10.1242/dev.02744

Development 134, 455-465 (2007)
Published by The Company of Biologists 2007
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Regulation of redox metabolism in the mouse oocyte and embryo

Rémi Dumollard1,2,*, Zoe Ward2, John Carroll2 and Michael R. Duchen2

1 Laboratoire de Biologie du Développement UMR 7009 CNRS/Paris VI, Observatoire, Station Zoologique, Villefranche sur Mer, 06230 France.
2 Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK.

* Author for correspondence (e-mail: remi.dumollard{at}obs-vlfr.fr)

Accepted 14 November 2006

Energy homeostasis of the oocyte is a crucial determinant of fertility. Following ovulation, the oocyte is exposed to the unique environment of the Fallopian tube, and this is reflected in a highly specialised biochemistry. The minute amounts of tissue available have made the physiological analysis of oocyte intermediary metabolism almost impossible. We have therefore used confocal imaging of mitochondrial and cytosolic redox state under a range of conditions to explore the oxidative metabolism of intermediary substrates. It has been known for some time that the early mouse embryo metabolises external pyruvate and lactate but not glucose to produce ATP. We now show at the level of single oocytes, that supplied glucose has no effect on the redox potential of the oocyte. Pyruvate is a cytosolic oxidant but a mitochondrial reductant, while lactate is a strong cytosolic reductant via the activity of lactate dehydrogenase. Unexpectedly, lactate-derived pyruvate appears to be diverted from mitochondrial oxidation. Our approach also reveals that the level of reduced glutathione (GSH) in the oocyte is maintained by glutathione reductase, which oxidises intracellular NADPH to reduce oxidised glutathione. Surprisingly, NADPH does not seem to be supplied by the pentose phosphate pathway in the unfertilised oocyte but rather by cytosolic NADP-dependent isocitrate dehydrogenase. Remarkably, we also found that the oxidant action of pyruvate impairs development, demonstrating the fundamental importance of redox state on early development.

Key words: Mouse, Oocyte, Autofluorescence, NAD(P)H, Oxido-reduction, Mitochondria






© The Company of Biologists Ltd 2007