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First published online 14 February 2007
doi: 10.1242/dev.02781
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1 II. Medizinische Klinik, Klinikum rechts der Isar, Technische
Universtität München, Ismaninger Str. 22, D-81675 Munich,
Germany.
2 Department of Ophthalmology, Martin-Luther-University Halle-Wittenberg,
Ernst-Grube-Strasse 40, D-06120 Halle/Saale, Germany.
3 Institute of Human Genetics, GSF-National Research Center for Environment and
Health, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany.
4 Department of Anatomy and Cell Biology, Martin-Luther-University
Halle-Wittenberg, Grosse Steinstrasse 52, D-06097 Halle/Saale, Germany.
Author for correspondence (e-mail:
hassan.nakhai{at}lrz.tum.de)
Accepted 14 December 2006
Basic helix-loop-helix (bHLH) transcription factors are important regulators of retinal neurogenesis. In the developing retina, proneural bHLH genes have highly defined expressions, which are influenced by pattern formation and cell-specification pathways. We report here that the tissue-specific bHLH transcription factor Ptf1a (also known as PTF1-p48) is expressed from embryonic day 12.5 of gestation (E12.5) to postnatal day 3 (P3) during retinogenesis in the mouse. Using recombination-based lineage tracing, we provide evidence that Ptf1a is expressed in precursors of amacrine and horizontal cells. Inactivation of Ptf1a in the developing retina led to differentiation arrest of amacrine and horizontal precursor cells in addition to partial transdifferentiation of Ptf1a-expressing precursor cells to ganglion cells. Analysis of late cell-type-specific markers revealed the presence of a small population of differentiated amacrine cells, whereas GABAergic and glycinergic amacrine cells, as well as horizontal cells, were completely missing in Ptf1a-knockout retinal explants. We conclude that Ptf1a contributes to the differentiation of horizontal cells and types of amacrine cells during mouse retinogenesis.
Key words: Amacrine cells, Cre-loxP method, Development, Ptf1a, Retina, Mouse
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