QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 7 March 2007
doi: 10.1242/dev.02834

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: dev.02834v1 134/8/1481    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Taneyhill, L. A. Articles by Bronner-Fraser, M. Search for Related Content PubMed PubMed Citation Articles by Taneyhill, L. A. Articles by Bronner-Fraser, M. Social Bookmarking                         What's this?

Snail2 directly represses cadherin6B during epithelial-to-mesenchymal transitions of the neural crest

Lisa A. Taneyhill^*, Edward G. Coles^* and Marianne Bronner-Fraser

Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

Author for correspondence (e-mail: mbronner{at}caltech.edu)

Accepted 5 February 2007

The neural crest, a transient population of migratory cells, forms the craniofacial skeleton and peripheral nervous system, among other derivatives in vertebrate embryos. The transcriptional repressor Snail2 is thought to be crucial for the epithelial-to-mesenchymal transition (EMT) that promotes neural crest delamination from the neural tube; however, little is known about its downstream targets. To this end, we depleted avian Snail2 in the premigratory neural crest using morpholino antisense oligonucleotides and examined effects on potential targets by quantitative PCR. Several dorsal neural tube genes were upregulated by alleviating Snail2 repression; moreover, the cell adhesion molecule cadherin6B was derepressed within 30 minutes of blocking Snail2 translation. Examination of the chick cadherin6B genomic sequence reveals that the regulatory region contains three pairs of clustered E boxes, representing putative Snail2 binding sites. Furthermore, in vivo and in vitro biochemical analyses demonstrate that Snail2 directly binds to these sites and regulates cadherin6B transcription. These results are the first to describe a direct target of Snail2 repression in vivo and in the context of the EMT that characterizes neural crest development.

Key words: Snail2 (Slug), cadherin6B, Neural crest, Epithelial-to-mesenchymal transitions, E boxes

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				C. Fournier-Thibault, C. Blavet, A. Jarov, F. Bajanca, S. Thorsteinsdottir, and J.-L. Duband Sonic Hedgehog Regulates Integrin Activity, Cadherin Contacts, and Cell Polarity to Orchestrate Neural Tube Morphogenesis J. Neurosci., October 7, 2009; 29(40): 12506 - 12520. [Abstract] [Full Text] [PDF]

				T. Sauka-Spengler and M. Bronner-Fraser Insights From a Sea Lamprey Into the Evolution of Neural Crest Gene Regulatory Network Biol. Bull., June 1, 2008; 214(3): 303 - 314. [Abstract] [Full Text] [PDF]