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First published online 28 November 2007
doi: 10.1242/dev.010744

Development 135, 123-131 (2008)
Published by The Company of Biologists 2008
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A novel promoter-tethering element regulates enhancer-driven gene expression at the bithorax complex in the Drosophila embryo

Omar S. Akbari1, Esther Bae2, Holly Johnsen3, Alfred Villaluz1, Debbie Wong1 and Robert A. Drewell3,*

1 Biology Department M/S 314, University of Nevada, Reno, 1664 N. Virginia Street, Reno, NV 89557, USA.
2 College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E 2nd Street, Pomona, CA 91766, USA.
3 Biology Department, Harvey Mudd College, 301 Platt Boulevard, Claremont, CA 91711, USA.

* Author for correspondence (e-mail: drewell{at}hmc.edu)

Accepted 10 October 2007

A key question in our understanding of the cis-regulation of gene expression during embryonic development has been the molecular mechanism that directs enhancers to specific promoters within a gene complex. Promoter competition and insulators are thought to play a role in regulating these interactions. In the bithorax complex of Drosophila, the IAB5 enhancer is located 55 kb 3' of the Abdominal-B (Abd-B) promoter and 48 kb 5' of the abdominal-A (abd-A) promoter. Although roughly equidistant from the two promoters, IAB5 specifically interacts only with the Abdominal-B promoter, even though the enhancer and promoter are separated by at least two insulators. Here we demonstrate that a 255 bp element, located 40 bp 5' of the Abd-B transcriptional start site, has a novel cis-regulatory activity as it is able to tether IAB5 to the Abd-B promoter in transgenic embryos. The tethering element is sufficient to direct IAB5 to an ectopic promoter in competition assays. Deletion of the promoter-tethering element results in the redirection of enhancer-driven gene expression on transgenes. Taken together, these results provide evidence that specific long-range enhancer-promoter interactions in the bithorax complex are regulated by a tethering element 5' of the Abd-B promoter. We discuss a bioinformatic analysis of the tethering element across different Drosophila species and a possible molecular mechanism by which this element functions. We also examine existing evidence that this novel class of cis-regulatory elements might regulate enhancer-promoter specificity at other gene complexes.

Key words: cis-regulation, Enhancer, Drosophila, Bithorax, Abdominal-B, Promoter




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