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First published online 28 May 2008
doi: 10.1242/dev.022020

Development 135, 2289-2299 (2008)
Published by The Company of Biologists 2008
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Localised axial progenitor cell populations in the avian tail bud are not committed to a posterior Hox identity

Michael J. McGrew1, Adrian Sherman1, Simon G. Lillico1, Fiona M. Ellard2, Pippa A. Radcliffe2, Hazel J. Gilhooley1, Kyriacos A. Mitrophanous2, Noemí Cambray3, Valerie Wilson3,* and Helen Sang1,*,{dagger}

1 The Roslin Institute and Royal Dick School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, UK.
2 Oxford Biomedica (UK) Ltd, Medawar Centre, Oxford Science Park, Oxford, UK.
3 Institute for Stem Cell Research, MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, UK.

{dagger} Author for correspondence (e-mail: helen.sang{at}bbsrc.ac.uk)

Accepted 29 April 2008

The outgrowth of the vertebrate tail is thought to involve the proliferation of regionalised stem/progenitor cell populations formed during gastrulation. To follow these populations over extended periods, we used cells from GFP-positive transgenic chick embryos as a source for donor tissue in grafting experiments. We determined that resident progenitor cell populations are localised in the chicken tail bud. One population, which is located in the chordoneural hinge (CNH), contributes descendants to the paraxial mesoderm, notochord and neural tube, and is serially transplantable between embryos. A second population of mesodermal progenitor cells is located in a separate dorsoposterior region of the tail bud, and a corresponding population is present in the mouse tail bud. Using heterotopic transplantations, we show that the fate of CNH cells depends on their environment within the tail bud. Furthermore, we show that the anteroposterior identity of tail bud progenitor cells can be reset by heterochronic transplantation to the node region of gastrula-stage chicken embryos.

Key words: Progenitor cell, Tail bud, Chordoneural hinge, Hox genes, Transgenic chicken


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A tail of axial progenitors

Development 2008 135: e1305. [Full Text]  





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