QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online September 5, 2008
doi: 10.1242/10.1242/dev.024406

This Article Figures Only Full Text Full Text (PDF) Supplementary Material Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, H. Articles by Peng, J. Search for Related Content PubMed PubMed Citation Articles by Huang, H. Articles by Peng, J. Social Bookmarking                         What's this?

Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis

Honghui Huang^1,5, Hua Ruan^1,5,*, Meng Yuan Aw^1,*, Alamgir Hussain^1,*, Lin Guo^1,5,*, Chuan Gao⁵, Feng Qian¹, Thomas Leung², Haiwei Song³, David Kimelman⁶, Zilong Wen^4, and Jinrong Peng^1,

¹ Laboratory of Functional Genomics, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673.
² Laboratory of Neural Differentiation and Degeneration, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673.
³ Laboratory of Translation Termination and Messenger RNA Decay, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673.
⁴ Laboratory of Molecular and Developmental Immunology, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673.
⁵ Department of Biological Sciences, National University of Singapore, Singapore 117543.
⁶ Department of Biochemistry, University of Washington, Seattle, WA 98195-7350, USA.

Authors for correspondence: (e-mails: zilong{at}ust.hk; pengjr{at}imcb.a-star.edu.sg)

Accepted 1 August 2008

Mesodermal tissues produce various inductive signals essential for morphogenesis of endodermal organs. However, little is known about how the spatial relationship between the mesodermal signal-producing cells and their target endodermal organs is established during morphogenesis. Here, we report that a mutation in the zebrafish myosin phosphatase targeting subunit 1 (mypt1) gene causes abnormal bundling of actin filaments and disorganization of lateral plate mesoderm (LPM) and endoderm cells. As a result, the coordination between mesoderm and endoderm cell movements is disrupted. Consequently, the two stripes of Bmp2a-expressing cells in the LPM fail to align in a V-shaped pocket sandwiching the liver primordium. Mispositioning Bmp2a-producing cells with respect to the liver primordium leads to a reduction in hepatoblast proliferation and final abortion of hepatoblasts by apoptosis, causing the liverless phenotype. Our results demonstrate that Mypt1 mediates coordination between mesoderm and endoderm cell movements in order to carefully position the liver primordium such that it receives a Bmp signal that is essential for liver formation in zebrafish.

Key words: Liver organogenesis, Mypt1, Bmp, Zebrafish

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in Development:

Liver organogenesis: location matters

Development 2008 135: e1901. [Full Text]  

This article has been cited by other articles:

				D. C. Weiser, R. H. Row, and D. Kimelman Rho-regulated Myosin phosphatase establishes the level of protrusive activity required for cell movements during zebrafish gastrulation Development, July 15, 2009; 136(14): 2375 - 2384. [Abstract] [Full Text] [PDF]