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First published online 5 December 2007
doi: 10.1242/dev.011445

Development 135, 259-269 (2008)
Published by The Company of Biologists 2008
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Maternally derived FILIA-MATER complex localizes asymmetrically in cleavage-stage mouse embryos

Mami Ohsugi, Ping Zheng, Boris Baibakov, Lei Li and Jurrien Dean*

Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA.

* Author for correspondence (e-mail: jurrien{at}helix.nih.gov)

Accepted 4 October 2007

Initial cell lineages that presage the inner cell mass and extra-embryonic trophectoderm are established when eight blastomeres compact to form polarized morulae in preimplantation mouse development. FILIA has been identified as a binding partner to MATER (maternal antigen that embryos require; also known as NLRP5), which is encoded by a maternal effect gene. Products of each gene are detected in growing oocytes and, although transcripts are degraded before fertilization, the cognate proteins persist in early blastocysts. The two proteins co-localize to the cytocortex of ovulated eggs, where the stability of FILIA is dependent on the presence of MATER. After fertilization, FILIA-MATER complexes become asymmetrically restricted in the apical cytocortex of two-cell embryos due to their absence in regions of cell-cell contact. This asymmetry is reversible upon disaggregation of blastomeres of the two- and four-cell embryo. Each protein persists in cells of the preimplantation embryo, but the continuous cell-cell contact of `inner' cells of the morulae seemingly precludes formation of the subcortical FILIA-MATER complex and results in cell populations that are marked by its presence (`outer') or absence (`inner'). Thus, the FILIA-MATER complex provides a molecular marker of embryonic cell lineages, but it remains to be determined if the molecular asymmetry established after the first cell division plays a role in cell fate determinations in the early mouse embryo. If so, the plasticity of the FILIA-MATER complex localization may reflect the regulative nature of preimplantation mouse development.

Key words: Preimplantation mouse development, Maternal effect genes, FILIA-MATER complex, Apical cytocortical polarization in blastomeres






© The Company of Biologists Ltd 2008