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First published online 2 October 2008
doi: 10.1242/dev.028118

Development 135, 3587-3597 (2008)
Published by The Company of Biologists 2008
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PTEN deficiency causes dyschondroplasia in mice by enhanced hypoxia-inducible factor 1{alpha} signaling and endoplasmic reticulum stress

Guan Yang, Qiang Sun, Yan Teng, Fangfei Li, Tujun Weng and Xiao Yang*

State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, 20 Dongdajie, Beijing 100071, China.

* Author for correspondence (e-mail: yangx{at}nic.bmi.ac.cn)

Accepted 10 September 2008

Chondrocytes within the growth plates acclimatize themselves to a variety of stresses that might otherwise disturb cell fate. The tumor suppressor PTEN (phosphatase and tensin homolog deleted from chromosome 10) has been implicated in the maintenance of cell homeostasis. However, the functions of PTEN in regulating chondrocytic adaptation to stresses remain largely unknown. In this study, we have created chondrocyte-specific Pten knockout mice (Ptenco/co;Col2a1-Cre) using the Cre-loxP system. Following AKT activation, Pten mutant mice exhibited dyschondroplasia resembling human enchondroma. Cartilaginous nodules originated from Pten mutant resting chondrocytes that suffered from impaired proliferation and differentiation, and this was coupled with enhanced endoplasmic reticulum (ER) stress. We further found that ER stress in Pten mutant chondrocytes only occurred under hypoxic stress, characterized by an upregulation of unfolded protein response-related genes as well as an engorged and fragmented ER in which collagens were trapped. An upregulation of hypoxia-inducible factor 1{alpha} (HIF1{alpha}) and downstream targets followed by ER stress induction was also observed in Pten mutant growth plates and in cultured chondrocytes, suggesting that PI3K/AKT signaling modulates chondrocytic adaptation to hypoxic stress via regulation of the HIF1{alpha} pathway. These data demonstrate that PTEN function in chondrocytes is essential for their adaptation to stresses and for the inhibition of dyschondroplasia.

Key words: PTEN, Dyschondroplasia, ER stress, HIF1{alpha}, Knockout mouse




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
G. Yang, Q. Sun, Y. Teng, F. Li, T. Weng, and X. Yang
PTEN deficiency causes dyschondroplasia in mice by enhanced hypoxia-inducible factor 1 signaling and endoplasmic reticulum stress
J. Cell Sci., November 1, 2008; 121(21): e2107 - e2107.
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