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First published online 16 January 2008
doi: 10.1242/dev.015339

Development 135, 717-727 (2008)
Published by The Company of Biologists 2008
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Inactivation of nuclear Wnt-β-catenin signaling limits blastocyst competency for implantation

Huirong Xie1, Susanne Tranguch2, Xiangxu Jia1, Hao Zhang1, Sanjoy K. Das1,3, Sudhansu K. Dey1,2,4, Calvin J. Kuo5 and Haibin Wang1,*

1 Departments of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.
2 Departments of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
3 Departments of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
4 Departments of Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA.
5 Department of Medicine, Stanford University School of Medicine, Center for Clinical Sciences Research 3100, 269 Campus Drive, Stanford, CA 94305, USA.

* Author for correspondence (e-mail: h.wang{at}vanderbilt.edu)

Accepted 5 December 2007

The activation of the blastocyst, a process by which it gains competency to attach with the receptive uterus, is a prerequisite for successful implantation. However, the molecular basis of blastocyst activation remains largely unexplored. Combining molecular, pharmacological and physiological approaches, we show here that silencing of Wnt-β-catenin signaling in mice does not adversely affect the development of preimplantation embryos to blastocysts and uterine preparation for receptivity, but, remarkably, blocks blastocyst competency to implantation. Using the physiologically relevant delayed implantation model and trophoblast stem cells in culture, we further demonstrate that a coordinated activation of canonical Wnt-β-catenin signaling with attenuation of the non-canonical Wnt-RhoA signaling pathway ensures blastocyst competency to implantation. These findings constitute novel evidence that Wnt signaling is at least one pathway that determines blastocyst competency for implantation.

Key words: Blastocyst activation, Implantation, Wnt, β-catenin, Mouse






© The Company of Biologists Ltd 2008