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First published online 30 January 2008
doi: 10.1242/dev.015552

Development 135, 983-993 (2008)
Published by The Company of Biologists 2008
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DEPS-1 promotes P-granule assembly and RNA interference in C. elegans germ cells

Caroline A. Spike1,*, Jason Bader1,{dagger}, Valerie Reinke2 and Susan Strome1,{ddagger},§

1 Department of Biology, Indiana University, Bloomington, IN 47405, USA.
2 Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.

§ Author for correspondence (e-mail: strome{at}biology.ucsc.edu)

Accepted 18 December 2007

P granules are germ-cell-specific cytoplasmic structures containing RNA and protein, and required for proper germ cell development in C. elegans. PGL-1 and GLH-1 were previously identified as critical components of P granules. We have identified a new P-granule-associated protein, DEPS-1, the loss of which disrupts P-granule structure and function. DEPS-1 is required for the proper localization of PGL-1 to P granules, the accumulation of glh-1 mRNA and protein, and germ cell proliferation and fertility at elevated temperatures. In addition, DEPS-1 is required for RNA interference (RNAi) of germline-expressed genes, possibly because DEPS-1 promotes the accumulation of RDE-4, a dsRNA-binding protein required for RNAi. A genome wide analysis of gene expression in deps-1 mutant germ lines identified additional targets of DEPS-1 regulation, many of which are also regulated by the RNAi factor RDE-3. Our studies suggest that DEPS-1 is a key component of the P-granule assembly pathway and that its roles include promoting accumulation of some mRNAs, such as glh-1 and rde-4, and reducing accumulation of other mRNAs, perhaps by collaborating with RDE-3 to generate endogenous short interfering RNAs (endo-siRNAs).

Key words: Germ granules, RNAi, Gene expression


Related articles in Development:

New DEPS to P granule assembly and RNAi

Development 2008 135: e506. [Full Text]  



This article has been cited by other articles:


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C. Spike, N. Meyer, E. Racen, A. Orsborn, J. Kirchner, K. Kuznicki, C. Yee, K. Bennett, and S. Strome
Genetic Analysis of the Caenorhabditis elegans GLH Family of P-Granule Proteins
Genetics, April 1, 2008; 178(4): 1973 - 1987.
[Abstract] [Full Text] [PDF]


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