QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 26 March 2008
doi: 10.1242/dev.012179

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: dev.012179v1 135/9/1615    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Teng, L. Articles by Labosky, P. A. Search for Related Content PubMed PubMed Citation Articles by Teng, L. Articles by Labosky, P. A. Social Bookmarking                         What's this?

Requirement for Foxd3 in the maintenance of neural crest progenitors

Lu Teng^1,*,, Nathan A. Mundell^2,4,, Audrey Y. Frist^3,4, Qiaohong Wang¹ and Patricia A. Labosky^1,2,3,4,

¹ Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA.
² Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232-0494, USA.
³ Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0494, USA.
⁴ Center for Stem Cell Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0494, USA.

Author for correspondence (e-mail: trish.labosky{at}vanderbilt.edu)

Accepted 28 February 2008

Understanding the molecular mechanisms of stem cell maintenance is crucial for the ultimate goal of manipulating stem cells for the treatment of disease. Foxd3 is required early in mouse embryogenesis; Foxd3^-/- embryos fail around the time of implantation, cells of the inner cell mass cannot be maintained in vitro, and blastocyst-derived stem cell lines cannot be established. Here, we report that Foxd3 is required for maintenance of the multipotent mammalian neural crest. Using tissue-specific deletion of Foxd3 in the neural crest, we show that Foxd3^flox/-; Wnt1-Cre mice die perinatally with a catastrophic loss of neural crest-derived structures. Cranial neural crest tissues are either missing or severely reduced in size, the peripheral nervous system consists of reduced dorsal root ganglia and cranial nerves, and the entire gastrointestinal tract is devoid of neural crest derivatives. These results demonstrate a global role for this transcriptional repressor in all aspects of neural crest maintenance along the anterior-posterior axis, and establish an unprecedented molecular link between multiple divergent progenitor lineages of the mammalian embryo.

Key words: Neural crest, Foxd3, Mouse embryo, Stem cell maintenance

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				C. Fournier-Thibault, C. Blavet, A. Jarov, F. Bajanca, S. Thorsteinsdottir, and J.-L. Duband Sonic Hedgehog Regulates Integrin Activity, Cadherin Contacts, and Cell Polarity to Orchestrate Neural Tube Morphogenesis J. Neurosci., October 7, 2009; 29(40): 12506 - 12520. [Abstract] [Full Text] [PDF]

				A. Calmont, S. Ivins, K. L. Van Bueren, I. Papangeli, V. Kyriakopoulou, W. D. Andrews, J. F. Martin, A. M. Moon, E. A. Illingworth, M. A. Basson, et al. Tbx1 controls cardiac neural crest cell migration during arch artery development by regulating Gbx2 expression in the pharyngeal ectoderm Development, September 15, 2009; 136(18): 3173 - 3183. [Abstract] [Full Text] [PDF]

				C. M. Drerup, H. M. Wiora, J. Topczewski, and J. A. Morris Disc1 regulates foxd3 and sox10 expression, affecting neural crest migration and differentiation Development, August 1, 2009; 136(15): 2623 - 2632. [Abstract] [Full Text] [PDF]

				L. Osorio, M.-A. Teillet, and M. Catala Role of noggin as an upstream signal in the lack of neuronal derivatives found in the avian caudal-most neural crest Development, May 15, 2009; 136(10): 1717 - 1726. [Abstract] [Full Text] [PDF]

				H. F. McGraw, A. Nechiporuk, and D. W. Raible Zebrafish Dorsal Root Ganglia Neural Precursor Cells Adopt a Glial Fate in the Absence of Neurogenin1 J. Neurosci., November 19, 2008; 28(47): 12558 - 12569. [Abstract] [Full Text] [PDF]