spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online July 10, 2009
doi: 10.1242/10.1242/dev.036517

Development 136, 2557-2565 (2009)
Published by The Company of Biologists 2009
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Zanet, J.
Right arrow Articles by Plaza, S.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zanet, J.
Right arrow Articles by Plaza, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Fascin is required for blood cell migration during Drosophila embryogenesis

Jennifer Zanet1,3, Brian Stramer2,*, Thomas Millard2,{dagger}, Paul Martin2, François Payre1,3,{ddagger} and Serge Plaza1,3,{ddagger}

1 Université de Toulouse, UPS, Centre de Biologie du Développement, Bâtiment 4R3, 118 route de Narbonne, F-31062 Toulouse, France.
2 University of Bristol, Departments of Biochemistry and Physiology & Pharmacology, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK.
3 CNRS, UMR5547, Centre de Biologie du Développement, F-31062 Toulouse, France.

{ddagger} Authors for correspondence (e-mails: payre{at}cict.fr; plaza{at}cict.fr)

Accepted 28 May 2009

Fascin is well characterized in vitro as an actin-bundling protein and its increased expression is correlated with the invasiveness of various cancers. However, the actual roles and regulation of Fascin in vivo remain elusive. Here we show that Fascin is required for the invasive-like migration of blood cells in Drosophila embryos. Fascin expression is highly regulated during embryonic development and, within the blood lineage, is specific to the motile subpopulation of cells, which comprises macrophage-like plasmatocytes. We show that Fascin is required for plasmatocyte migration, both as these cells undergo developmental dispersal and during an inflammatory response to epithelial wounding. Live analyses further demonstrate that Fascin localizes to, and is essential for the assembly of, dynamic actin-rich microspikes within plasmatocyte lamellae that polarize towards the direction of migration. We show that a regulatory serine of Fascin identified from in vitro studies is not required for in vivo cell motility, but is crucial for the formation of actin bundles within epithelial bristles. Together, these results offer a first glimpse into the mechanisms regulating Fascin function during normal development, which might be relevant for understanding the impact of Fascin in cancers.

Key words: Drosophila, Fascin (Singed), Hemocytes, Migration, Wound healing


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in Development:

Fascin-ating fly blood cell migration

Development 2009 136: e1503. [Full Text]  





© The Company of Biologists Ltd 2009