spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 15 July 2009
doi: 10.1242/dev.038596

Development 136, 2725-2733 (2009)
Published by The Company of Biologists 2009
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.038596v1
136/16/2725    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Google Scholar
Right arrow Articles by Meyerzon, M.
Right arrow Articles by Starr, D. A.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meyerzon, M.
Right arrow Articles by Starr, D. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

UNC-83 is a nuclear-specific cargo adaptor for kinesin-1-mediated nuclear migration

Marina Meyerzon, Heidi N. Fridolfsson, Nina Ly, Francis J. McNally and Daniel A. Starr*

Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.

* Author for correspondence (e-mail: dastarr{at}ucdavis.edu)

Accepted 27 May 2009

Intracellular nuclear migration is essential for many cellular events including fertilization, establishment of polarity, division and differentiation. How nuclei migrate is not understood at the molecular level. The C. elegans KASH protein UNC-83 is required for nuclear migration and localizes to the outer nuclear membrane. UNC-83 interacts with the inner nuclear membrane SUN protein UNC-84 and is proposed to connect the cytoskeleton to the nuclear lamina. Here, we show that UNC-83 also interacts with the kinesin-1 light chain KLC-2, as identified in a yeast two-hybrid screen and confirmed by in vitro assays. UNC-83 interacts with and recruits KLC-2 to the nuclear envelope in a heterologous tissue culture system. Additionally, analysis of mutant phenotypes demonstrated that both KLC-2 and the kinesin-1 heavy chain UNC-116 are required for nuclear migration. Finally, the requirement for UNC-83 in nuclear migration could be partially bypassed by expressing a synthetic outer nuclear membrane KLC-2::KASH fusion protein. Our data support a model in which UNC-83 plays a central role in nuclear migration by acting to bridge the nuclear envelope and as a kinesin-1 cargo-specific adaptor so that motor-generated forces specifically move the nucleus as a single unit.

Key words: Kinesin-1, Nuclear envelope, KASH proteins, SUN proteins, Nuclear migration, UNC-83


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JCBHome page
D. Razafsky and D. Hodzic
Bringing KASH under the SUN: the many faces of nucleo-cytoskeletal connections
J. Cell Biol., August 24, 2009; 186(4): 461 - 472.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. D. McGee, I. Stagljar, and D. A. Starr
KDP-1 is a nuclear envelope KASH protein required for cell-cycle progression
J. Cell Sci., August 15, 2009; 122(16): 2895 - 2905.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2009