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First published online 29 July 2009
doi: 10.1242/dev.038935

Development 136, 2913-2921 (2009)
Published by The Company of Biologists 2009
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Control of extracellular matrix assembly along tissue boundaries via Integrin and Eph/Ephrin signaling

Dörthe Jülich1, A. Paul Mould2, Ewa Koper2 and Scott A. Holley1,*

1 Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
2 Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester M13 9PT, UK.

* Author for correspondence (scott.holley{at}yale.edu)

Accepted 30 June 2009

Extracellular matrixes (ECMs) coat and subdivide animal tissues, but it is unclear how ECM formation is restricted to tissue surfaces and specific cell interfaces. During zebrafish somite morphogenesis, segmental assembly of an ECM composed of Fibronectin (FN) depends on the FN receptor Integrin {alpha}5β1. Using in vivo imaging and genetic mosaics, our studies suggest that incipient Itg{alpha}5 clustering along the nascent border precedes matrix formation and is independent of FN binding. Integrin clustering can be initiated by Eph/Ephrin signaling, with Ephrin reverse signaling being sufficient for clustering. Prior to activation, Itg{alpha}5 expressed on adjacent cells reciprocally and non-cell-autonomously inhibits spontaneous Integrin clustering and assembly of an ECM. Surface derepression of this inhibition provides a self-organizing mechanism for the formation and maintenance of ECM along the tissue surface. Within the tissue, interplay between Eph/Ephrin signaling, ligand-independent Integrin clustering and reciprocal Integrin inhibition restricts de novo ECM production to somite boundaries.

Key words: Eph, Ephrin, Fibronectin, Integrin, Morphogenesis, Zebrafish


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D. Julich, A. P. Mould, E. Koper, and S. A. Holley
Control of extracellular matrix assembly along tissue boundaries via Integrin and Eph/Ephrin signaling
J. Cell Sci., September 1, 2009; 122(17): e1707 - e1707.
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