spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 12 August 2009
doi: 10.1242/dev.036426

Development 136, 3067-3075 (2009)
Published by The Company of Biologists 2009
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.036426v1
136/18/3067    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Google Scholar
Right arrow Articles by Kwon, D.
Right arrow Articles by Kassis, J. A.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kwon, D.
Right arrow Articles by Kassis, J. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Enhancer-promoter communication at the Drosophila engrailed locus

Deborah Kwon1, Diane Mucci2, Kristofor K. Langlais1, Jeffrey L. Americo1,*, Sarah K. DeVido1, Yuzhong Cheng1 and Judith A. Kassis1,{dagger}

1 Laboratory of Molecular Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
2 Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.

{dagger} Author for correspondence (jkassis{at}mail.nih.gov)

Accepted 5 July 2009

Enhancers are often located many tens of kilobases away from the promoter they regulate, sometimes residing closer to the promoter of a neighboring gene. How do they know which gene to activate? We have used homing P[en] constructs to study the enhancer-promoter communication at the engrailed locus. Here we show that engrailed enhancers can act over large distances, even skipping over other transcription units, choosing the engrailed promoter over those of neighboring genes. This specificity is achieved in at least three ways. First, early acting engrailed stripe enhancers exhibit promoter specificity. Second, a proximal promoter-tethering element is required for the action of the imaginal disc enhancer(s). Our data suggest that there are two partially redundant promoter-tethering elements. Third, the long-distance action of engrailed enhancers requires a combination of the engrailed promoter and sequences within or closely linked to the promoter proximal Polycomb-group response elements. These data show that multiple mechanisms ensure proper enhancer-promoter communication at the Drosophila engrailed locus.

Key words: Promoter specificity, Regulatory DNA, Transcriptional control, Drosophila


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in Development:

Homing in on enhancer-promoter interactions

Development 2009 136: e1802. [Full Text]  



This article has been cited by other articles:


Home page
 DevelopmentHome page
M. Fujioka, X. Wu, and J. B. Jaynes
A chromatin insulator mediates transgene homing and very long-range enhancer-promoter communication
Development, September 15, 2009; 136(18): 3077 - 3087.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2009