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First published online August 21, 2009
doi: 10.1242/10.1242/dev.031492

Development 136, 3143-3152 (2009)
Published by The Company of Biologists 2009
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Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo

Cammon B. Arrington1 and H. Joseph Yost2,*

1 Division of Pediatric Cardiology, University of Utah, Salt Lake City, UT 84112, USA.
2 Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA.

* Author for correspondence (jyost{at}genetics.utah.edu)

Accepted 16 July 2009

One of the first steps in zebrafish heart and gut organogenesis is the migration of bilateral primordia to the midline to form cardiac and gut tubes. The mechanisms that regulate this process are poorly understood. Here we show that the proteoglycan syndecan 2 (Sdc2) expressed in the extra-embryonic yolk syncytial layer (YSL) acts locally at the YSL-embryo interface to direct organ primordia migration, and is required for fibronectin and laminin matrix assembly throughout the embryo. Surprisingly, neither endogenous nor exogenous sdc2 expressed in embryonic cells can compensate for knockdown of sdc2 in the YSL, indicating that Sdc2 expressed in extra-embryonic tissues is functionally distinct from Sdc2 in embryonic cells. The effects of sdc2 knockdown in the YSL can be rescued by extra-embryonic Sdc2 lacking an extracellular proteolytic cleavage (shedding) site, but not by extra-embryonic Sdc2 lacking extracellular glycosaminoglycan (GAG) addition sites, suggesting that distinct GAG chains on extra-embryonic Sdc2 regulate extracellular matrix assembly, cell migration and epithelial morphogenesis of multiple organ systems throughout the embryo.

Key words: Cardiac, Fibronectin, Gut, Heart, Proteoglycan, Syndecan, Zebrafish


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© The Company of Biologists Ltd 2009