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First published online 17 September 2009
doi: 10.1242/dev.041822

Development 136, 3393-3397 (2009)
Published by The Company of Biologists 2009
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Research Report

Spindle alignment is achieved without rotation after the first cell cycle in Drosophila embryonic neuroblasts

Elena Rebollo1, Mónica Roldán2 and Cayetano Gonzalez1,3,*

1 Cell Division Group, IRB-Barcelona, PCB, c/Baldiri Reixac 10-12, Barcelona, Spain.
2 Servei de Microscòpia, Universitat Autònoma de Barcelona, Edifici C, Campus de la UAB, Bellaterra, Barcelona, Spain.
3 Institució Catalana de Recerca i Estudis Avançats, Passeig Lluís Companys 23, Barcelona, Spain.

* Author for correspondence (gonzalez{at}irbbarcelona.org)

Accepted 21 August 2009

SUMMARY

Spindle alignment along the apicobasal polarity axis is mandatory for proper self-renewing asymmetric division in Drosophila neuroblasts (NBs). In embryonic NBs, spindles have been reported to assemble orthogonally to the polarity axis and later rotate to align with it. In larval NBs, spindles assemble directly aligned with the axis owing to the differential spatiotemporal control of the microtubule organising activity of their centrosomes. We have recorded embryonic NBs that express centrosome and microtubule reporters, from delamination up to the fourth cell cycle, by two-photon confocal microscopy, and have found that the switch between these two spindle orientation modes occurs in the second cell cycle of the NB, the first that follows delamination. Therefore, predetermined spindle orientation is not restricted to larval NBs. On the contrary, it actually applies to all but the first cell cycle of embryonic NBs.

Key words: Drosophila, Spindle alignment, Neuroblast, Asymmetric division


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Rotation-free neuroblast spindle alignment

Development 2009 136: e2003. [Full Text]  





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