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First published online January 23, 2009
doi: 10.1242/10.1242/dev.016816

Development 136, 563-574 (2009)
Published by The Company of Biologists 2009
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The cell migration molecule UNC-53/NAV2 is linked to the ARP2/3 complex by ABI-1

Kristopher L. Schmidt1,2, Nancy Marcus-Gueret1,2, Adetayo Adeleye1,2, Jordan Webber1, David Baillie2 and Eve G. Stringham1,2,*

1 Department of Biology, Trinity Western University, 7600 Glover Road, Langley, BC V2Y 1Y1, Canada.
2 Department of Molecular Biology and Biochemistry, 8888 University Drive, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.

* Author for correspondence (e-mail: stringha{at}twu.ca)

Accepted 9 December 2008

The shape changes that are required to position a cell to migrate or grow out in a particular direction involve a coordinated reorganization of the actin cytoskeleton. Although it is known that the ARP2/3 complex nucleates actin filament assembly, exactly how the information from guidance cues is integrated to elicit ARP2/3-mediated remodeling during outgrowth remains vague. Previous studies have shown that C. elegans UNC-53 and its vertebrate homolog NAV (Neuronal Navigators) are required for the migration of cells and neuronal processes. We have identified ABI-1 as a novel molecular partner of UNC-53/NAV2 and have found that a restricted calponin homology (CH) domain of UNC-53 is sufficient to bind ABI-1. ABI-1 and UNC-53 have an overlapping expression pattern, and display similar cell migration phenotypes in the excretory cell, and in mechanosensory and motoneurons. Migration defects were also observed after RNAi of proteins known to function with abi-1 in actin dynamics, including nck-1, wve-1 and arx-2. We propose that UNC-53/NAV2, through its CH domain, acts as a scaffold that links ABI-1 to the ARP2/3 complex to regulate actin cytoskeleton remodeling.

Key words: Cell migration, Axonal guidance, Cytoskeleton, ARP2/3 complex, C. elegans


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Home page
 J. Cell Sci.Home page
K. L. Schmidt, N. Marcus-Gueret, A. Adeleye, J. Webber, D. Baillie, and E. G. Stringham
The cell migration molecule UNC-53/NAV2 is linked to the ARP2/3 complex by ABI-1
J. Cell Sci., February 15, 2009; 122(4): e406 - e406.
[Full Text]


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