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A New Technique for the Cultivation of the Chick Embryo in vitro

Department of Anatomy and Embryology, University College, London

¹ Author's address: Department of Anatomy and Embryology, University College, London, U.K.

Received for publication 14 December 1954.

SUMMARY

The inaccessibility of the chick embryo in the egg has led to the invention of a large number of in vitro techniques for its cultivation and study. The simplest of these techniques consists of little more than pouring the entire egg contents into a suitable container and taking precautions against excessive evaporation or bacterial infection (e.g. Assheton, 1896; Schmidt, 1937; Vollmar, 1935; Romanoff, 1943). Although these methods assist observation of gross changes occurring in the embryo or its membranes, they are of little use to the embryologist who wishes to make detailed observations or operations. It is very difficult to determine the outline of, for example, a primitive streak, whilst the blastoderm still lies on the yolk; and any operation on the blastoderm is usually followed by a slow leakage of yolk through the wound, which ruins the preparation.

The most successful method devised hitherto for explanting the blastoderm in isolation from the yolk is that of Waddington (1932).

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