QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Selman, G. G. Search for Related Content PubMed Articles by Selman, G. G. Social Bookmarking                         What's this?

An Ultra-Violet Light Method for Producing Haploid Amphibian Embryos

Institute of Animal Genetics, Edinburgh

¹ Author's address: Institute of Animal Genetics, West Mains Road, Edinburgh 9, U.K.

Received for publication 27 May 1958.

SUMMARY

For several years in this laboratory, ultra-violet light has been used to produce haploid amphibian embryos, sometimes to provide haploid nuclei for transplantation experiments, as in Pantelouris & Jacob (1958). The ultra-violet method gives a high proportion of haploids, is very simple and speedy to carry out and involves the use of only inexpensive apparatus. Our method and results are described here to help others choose between this and alternative methods such as the use of toluidine blue by Briggs (1952), or X-rays by Lehman (1955) and Rugh (1939). Other methods are reviewed in Fankhauser (1937) and Drebinger (1951).

Eggs were taken from the oviducts of newt females and were placed close together in groups on cellophane squares to which they adhered by their jelly coats so that their animal surfaces were uppermost. The cellophane squares of unfertilized eggs were then placed in Petri dishes in air kept moist with a piece of damp filter paper at one side.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?