spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Etkin, L. D.
Right arrow Articles by Pearman, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Etkin, L. D.
Right arrow Articles by Pearman, B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 99, Issue 1 15-23, Copyright © 1987 by Company of Biologists

JOURNAL ARTICLES

Distribution, expression and germ line transmission of exogenous DNA sequences following microinjection into Xenopus laevis eggs

LD Etkin and B Pearman
Department of Genetics, University of Texas M.D. Anderson Hospital and Tumor Institute, Houston 77030.

We analysed the fate, expression and germ line transmission of exogenous DNA which was microinjected into fertilized eggs of Xenopus laevis. DNA was injected into fertilized eggs within 1 h following fertilization. The injected DNA was dispersed around the site of injection and became localized to cleavage nuclei by stage 6. Injected DNA persisted in the tissues of 6- to 8-month-old frogs and exhibited a mosaic pattern of distribution with regard to the presence or absence and copy number between different tissues. We detected the exogenous DNA sequences in 60% of injected frogs. Restriction digestion analysis of this DNA suggested that it is not rearranged and was organized as head-to-tail multimers. The copy number varied from 2 to 30 copies/cell in various tissues of the same frog. Plasmid pSV2CAT which contains the prokaryotic gene coding for chloramphenicol acetyl transferase (CAT) enzyme linked to the SV40 early gene promoter was expressed in 50% of the animals containing the gene. The pattern of expression, however, varied between different animals and could not be correlated with copy number. We also showed that the exogenous DNA sequences were transmitted through the male germ line and that each offspring contained the gene integrated into a different region of the genome.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
A. Terskikh, A. Fradkov, G. Ermakova, A. Zaraisky, P. Tan, A. V. Kajava, X. Zhao, S. Lukyanov, M. Matz, S. Kim, et al.
"Fluorescent Timer": Protein That Changes Color with Time
Science, November 24, 2000; 290(5496): 1585 - 1588.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Huang, N. Marsh-Armstrong, and D. D. Brown
Metamorphosis is inhibited in transgenic Xenopus laevis tadpoles that overexpress type III deiodinase
PNAS, February 2, 1999; 96(3): 962 - 967.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Arnone, L. Bogarad, A Collazo, C. Kirchhamer, R. Cameron, J. Rast, A Gregorians, and E. Davidson
Green Fluorescent Protein in the sea urchin: new experimental approaches to transcriptional regulatory analysis in embryos and larvae
Development, January 11, 1997; 124(22): 4649 - 4659.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
X Li and L. Etkin
Xenopus nuclear factor 7 (xnf7) possesses an NLS that functions efficiently in both oocytes and embryos
J. Cell Sci., January 6, 1993; 105(2): 389 - 395.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
J. A. Sanchez, D. Marek, and L. J. Wangh
The efficiency and timing of plasmid DNA replication in Xenopus eggs: correlations to the extent of prior chromatin assembly
J. Cell Sci., December 1, 1992; 103(4): 907 - 918.
[Abstract] [PDF]


© The Company of Biologists Ltd 1987