spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 8 Oct 2003
doi: 10.1242/dev.00790

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.00790v1
130/23/5717    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsou, M.-F. B.
Right arrow Articles by Rose, L. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsou, M.-F. B.
Right arrow Articles by Rose, L. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

LET-99 opposes G{alpha}/GPR signaling to generate asymmetry for spindle positioning in response to PAR and MES-1/SRC-1 signaling


Meng-Fu Bryan Tsou, Adam Hayashi, and Lesilee S. Rose*
* Author for correspondence (e-mail: lsrose{at}ucdavis.edu)

G-protein signaling plays important roles in asymmetric cell division. In C. elegans embryos, homologs of receptor-independent G protein activators, GPR-1 and GPR-2 (GPR-1/2), function together with G{alpha} (GOA-1 and GPA-16) to generate asymmetric spindle pole elongation during divisions in the P lineage. Although G{alpha} is uniformly localized at the cell cortex, the cortical localization of GPR-1/2 is asymmetric in dividing P cells. In this report, we show that the asymmetry of GPR-1/2 localization depends on PAR-3 and its downstream intermediate LET-99. Furthermore, in addition to its involvement in spindle elongation, G{alpha} is required for the intrinsically programmed nuclear rotation event that orients the spindle in the one-cell. LET-99 functions antagonistically to the G{alpha}/GPR-1/2 signaling pathway, providing an explanation for how G{alpha}-dependent force is regulated asymmetrically by PAR polarity cues during both nuclear rotation and anaphase spindle elongation. In addition, G{alpha} and LET-99 are required for spindle orientation during the extrinsically polarized division of EMS cells. In this cell, both GPR-1/2 and LET-99 are asymmetrically localized in response to the MES-1/SRC-1 signaling pathway. Their localization patterns at the EMS/P2 cell boundary are complementary, suggesting that LET-99 and G{alpha}/GPR-1/2 signaling function in opposite ways during this cell division as well. These results provide insight into how polarity cues are transmitted into specific spindle positions in both extrinsic and intrinsic pathways of asymmetric cell division.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
H. Zhang, J. M. Squirrell, and J. G. White
RAB-11 Permissively Regulates Spindle Alignment by Modulating Metaphase Microtubule Dynamics in Caenorhabditis elegans Early Embryos
Mol. Biol. Cell, June 1, 2008; 19(6): 2553 - 2565.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. K. Baruni, E. M. Munro, and G. von Dassow
Cytokinetic furrowing in toroidal, binucleate and anucleate cells in C. elegans embryos
J. Cell Sci., February 1, 2008; 121(3): 306 - 316.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Zhang, A. R. Skop, and J. G. White
Src and Wnt signaling regulate dynactin accumulation to the P2-EMS cell border in C. elegans embryos
J. Cell Sci., January 15, 2008; 121(2): 155 - 161.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. Kimura and S. Onami
Local cortical pulling-force repression switches centrosomal centration and posterior displacement in C. elegans
J. Cell Biol., December 31, 2007; 179(7): 1347 - 1354.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J.-C. Wu and L. S. Rose
PAR-3 and PAR-1 Inhibit LET-99 Localization to Generate a Cortical Band Important for Spindle Positioning in Caenorhabditis elegans Embryos
Mol. Biol. Cell, November 1, 2007; 18(11): 4470 - 4482.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. Couwenbergs, J.-C. Labbe, M. Goulding, T. Marty, B. Bowerman, and M. Gotta
Heterotrimeric G protein signaling functions with dynein to promote spindle positioning in C. elegans
J. Cell Biol., October 8, 2007; 179(1): 15 - 22.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. B. Goulding, J. C. Canman, E. N. Senning, A. H. Marcus, and B. Bowerman
Control of nuclear centration in the C. elegans zygote by receptor-independent G{alpha} signaling and myosin II
J. Cell Biol., September 24, 2007; 178(7): 1177 - 1191.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. Strauss, R. J. Adams, and N. Papalopulu
A default mechanism of spindle orientation based on cell shape is sufficient to generate cell fate diversity in polarised Xenopus blastomeres
Development, October 1, 2006; 133(19): 3883 - 3893.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Afshar, F. S. Willard, K. Colombo, D. P. Siderovski, and P. Gonczy
Cortical localization of the G{alpha} protein GPA-16 requires RIC-8 function during C. elegans asymmetric cell division
Development, October 15, 2005; 132(20): 4449 - 4459.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J.-C. Labbe, E. K. McCarthy, and B. Goldstein
The forces that position a mitotic spindle asymmetrically are tethered until after the time of spindle assembly
J. Cell Biol., October 25, 2004; 167(2): 245 - 256.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. C. Bergmann, M. Lee, B. Robertson, M.-F. B. Tsou, L. S. Rose, and W. B. Wood
Embryonic handedness choice in C. elegans involves the G{alpha} protein GPA-16
Development, December 1, 2003; 130(23): 5731 - 5740.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003