spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 21 Jan 2004
doi: 10.1242/dev.00975

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.00975v1
131/4/829    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Milewski, R. C.
Right arrow Articles by Epstein, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Milewski, R. C.
Right arrow Articles by Epstein, J. A.

Research article

Identification of minimal enhancer elements sufficient for Pax3 expression in neural crest and implication of Tead2 as a regulator of Pax3


Rita C. Milewski, Neil C. Chi, Jun Li, Christopher Brown, Min Min Lu, and Jonathan A. Epstein*
* Author for correspondence (e-mail: epsteinj{at}mail.med.upenn.edu)

Pax3 is a transcription factor that is required by Pre-migratory neural crest cells give rise to the peripheral nervous system, melanocytes, some vascular smooth muscle, and numerous other derivatives. These cells require the transcription factor Pax3, and both mice and humans with Pax3 deficiency exhibit neural crest-related developmental defects. Pax3 is also expressed in the dorsal neural tube, and by myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Molecular pathways that regulate Pax3 expression in the roof plate probably represent early upstream signals in neural crest induction. We have identified an enhancer region in the Pax3 genomic locus that is sufficient to recapitulate expression in neural crest precursors in transgenic mice. We show that Tead2, a member of the Tead box family of transcription factors, binds to a neural crest enhancer and activates Pax3 expression. Tead2, and its co-activator YAP65, are co-expressed with Pax3 in the dorsal neural tube, and mutation of the Tead2 binding site in the context of Pax3 transgenic constructs abolishes neural expression. In addition, a Tead2-Engrailed fusion protein is able to repress retinoic acid-induced Pax3 expression in P19 cells and in vivo. These results suggest that Tead2 is an endogenous activator of Pax3 in neural crest.


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. Sawada, H. Kiyonari, K. Ukita, N. Nishioka, Y. Imuta, and H. Sasaki
Redundant Roles of Tead1 and Tead2 in Notochord Development and the Regulation of Cell Proliferation and Survival
Mol. Cell. Biol., May 15, 2008; 28(10): 3177 - 3189.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
T. Yoshida
MCAT Elements and the TEF-1 Family of Transcription Factors in Muscle Development and Disease
Arterioscler. Thromb. Vasc. Biol., January 1, 2008; 28(1): 8 - 17.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
P. G. Engstrom, S. J. Ho Sui, O. Drivenes, T. S. Becker, and B. Lenhard
Genomic regulatory blocks underlie extensive microsynteny conservation in insects
Genome Res., December 1, 2007; 17(12): 1898 - 1908.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. D. Kulman, J. E. Harris, L. Xie, and E. W. Davie
Proline-rich Gla protein 2 is a cell-surface vitamin K-dependent protein that binds to the transcriptional coactivator Yes-associated protein
PNAS, May 22, 2007; 104(21): 8767 - 8772.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. E. Creemers, L. B. Sutherland, J. McAnally, J. A. Richardson, and E. N. Olson
Myocardin is a direct transcriptional target of Mef2, Tead and Foxo proteins during cardiovascular development
Development, November 1, 2006; 133(21): 4245 - 4256.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Zhao, G. Caretti, S. Mitchell, W. L. McKeehan, A. L. Boskey, L. M. Pachman, V. Sartorelli, and E. P. Hoffman
Fgfr4 Is Required for Effective Muscle Regeneration in Vivo: DELINEATION OF A MyoD-Tead2-Fgfr4 TRANSCRIPTIONAL PATHWAY
J. Biol. Chem., January 6, 2006; 281(1): 429 - 438.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. M. Morin-Kensicki, B. N. Boone, M. Howell, J. R. Stonebraker, J. Teed, J. G. Alb, T. R. Magnuson, W. O'Neal, and S. L. Milgram
Defects in Yolk Sac Vasculogenesis, Chorioallantoic Fusion, and Embryonic Axis Elongation in Mice with Targeted Disruption of Yap65
Mol. Cell. Biol., January 1, 2006; 26(1): 77 - 87.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Sawada, Y. Nishizaki, H. Sato, Y. Yada, R. Nakayama, S. Yamamoto, N. Nishioka, H. Kondoh, and H. Sasaki
Tead proteins activate the Foxa2 enhancer in the node in cooperation with a second factor
Development, November 1, 2005; 132(21): 4719 - 4729.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004