spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 21 Jan 2004
doi: 10.1242/dev.00989

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.00989v1
131/4/863    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, J. H.
Right arrow Articles by Wieschaus, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, J. H.
Right arrow Articles by Wieschaus, E.

Research article

src64 and tec29 are required for microfilament contraction during Drosophila cellularization


Jeffrey H. Thomas and Eric Wieschaus*
* Author for correspondence (e-mail: ewieschaus{at}princeton.edu)

Formation of the Drosophila cellular blastoderm involves both membrane invagination and cytoskeletal regulation. Mutations in src64 and tec29 reveal a novel role for these genes in controlling contraction of the actin-myosin microfilament ring during this process. Although membrane invagination still proceeds in mutant embryos, its depth is not uniform, and basal closure of the cells does not occur during late cellularization. Double-mutant analysis between scraps, a mutation in anillin that eliminates microfilament rings, and bottleneck suggests that microfilaments can still contract even though they are not organized into rings. However, the failure of rings to contract in the src64 bottleneck double mutant suggests that src64 is required for microfilament ring contraction even in the absence of Bottleneck protein. Our results suggest that src64-dependent microfilament ring contraction is resisted by Bottleneck to create tension and coordinate membrane invagination during early cellularization. The absence of Bottleneck during late cellularization allows src64-dependent microfilament ring constriction to drive basal closure.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
A. M. Sokac and E. Wieschaus
Zygotically controlled F-actin establishes cortical compartments to stabilize furrows during Drosophila cellularization
J. Cell Sci., June 1, 2008; 121(11): 1815 - 1824.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
V. Chandrasekaran and S. K. Beckendorf
Tec29 controls actin remodeling and endoreplication during invagination of the Drosophila embryonic salivary glands
Development, August 1, 2005; 132(15): 3515 - 3524.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. M. Field, M. Coughlin, S. Doberstein, T. Marty, and W. Sullivan
Characterization of anillin mutants reveals essential roles in septin localization and plasma membrane integrity
Development, June 15, 2005; 132(12): 2849 - 2860.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. F. Straight, C. M. Field, and T. J. Mitchison
Anillin Binds Nonmuscle Myosin II and Regulates the Contractile Ring
Mol. Biol. Cell, January 1, 2005; 16(1): 193 - 201.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004