The fully linked HTML version of this article has now been published.
Development ePress online publication date 26 May 2004
doi: 10.1242/dev.01170
Research article
Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin
Young J. Yoon
and
Kimberly L. Mowry*
* Author for correspondence (e-mail: kimberly mowry{at}brown.edu)
RNA localization is a key mechanism for generating cell and developmental polarity in a wide variety of organisms. We have performed studies to investigate a role for the Xenopus homolog of the double-stranded RNA-binding protein, Staufen, in RNA localization during oogenesis. We have found that Xenopus Staufen (XStau) is present in a ribonucleoprotein complex, and associates with both a kinesin motor protein and vegetally localized RNAs Vg1 and VegT. A functional role for XStau was revealed through expression of a dominant-negative version that blocks localization of Vg1 RNA in vivo. Our results suggest a central role for XStau in RNA localization in Xenopus oocytes, and provide evidence that Staufen is a conserved link between specific mRNAs and the RNA localization machinery.

CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati
Twitter What's this?
This article has been cited by other articles:

|
 |

|
 |
 
P. K. Arthur, M. Claussen, S. Koch, K. Tarbashevich, O. Jahn, and T. Pieler
Participation of Xenopus Elr-type Proteins in Vegetal mRNA Localization during Oogenesis
J. Biol. Chem.,
July 24, 2009;
284(30):
19982 - 19992.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Git, R. Allison, E. Perdiguero, A. R. Nebreda, E. Houliston, and N. Standart
Vg1RBP phosphorylation by Erk2 MAP kinase correlates with the cortical release of Vg1 mRNA during meiotic maturation of Xenopus oocytes
RNA,
June 1, 2009;
15(6):
1121 - 1133.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Heinrich and J. O. Deshler
RNA localization to the Balbiani body in Xenopus oocytes is regulated by the energy state of the cell and is facilitated by kinesin II
RNA,
April 1, 2009;
15(4):
524 - 536.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. G. Thomas, L. J. M. Tosar, M. A. Desbats, C. C. Leishman, and G. L. Boccaccio
Mammalian Staufen 1 is recruited to stress granules and impairs their assembly
J. Cell Sci.,
February 15, 2009;
122(4):
563 - 573.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Yamaguchi, R. Oohinata, T. Naiki, and K. Irie
Stau1 negatively regulates myogenic differentiation in C2C12 cells.
Genes Cells,
June 1, 2008;
13(6):
583 - 592.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. A. Lewis, J. A. Gagnon, and K. L. Mowry
PTB/hnRNP I Is Required for RNP Remodeling during RNA Localization in Xenopus Oocytes
Mol. Cell. Biol.,
January 15, 2008;
28(2):
678 - 686.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. Oberman, K. Rand, Y. Maizels, A. M. Rubinstein, and J. K. Yisraeli
VICKZ proteins mediate cell migration via their RNA binding activity
RNA,
September 1, 2007;
13(9):
1558 - 1569.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Jambhekar and J. L. DeRisi
Cis-acting determinants of asymmetric, cytoplasmic RNA transport
RNA,
May 1, 2007;
13(5):
625 - 642.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. Scholz, P. McDermott, M. Garnovskaya, T. N. Gallien, S. Huettelmaier, C. DeRienzo, and G. Cooper IV
Microtubule-associated protein-4 (MAP-4) inhibits microtubule-dependent distribution of mRNA in isolated neonatal cardiocytes
Cardiovasc Res,
August 1, 2006;
71(3):
506 - 516.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. G. Gindhart
Towards an understanding of kinesin-1 dependent transport pathways through the study of protein-protein interactions
Brief Funct Genomic Proteomic,
March 1, 2006;
5(1):
74 - 86.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. CZAPLINSKI and I. W. MATTAJ
40LoVe interacts with Vg1RBP/Vera and hnRNP I in binding the Vg1-Localization Element
RNA,
February 1, 2006;
12(2):
213 - 222.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Goetze, F. Tuebing, Y. Xie, M. M. Dorostkar, S. Thomas, U. Pehl, S. Boehm, P. Macchi, and M. A. Kiebler
The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis
J. Cell Biol.,
January 17, 2006;
172(2):
221 - 231.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Shav-Tal and R. H. Singer
RNA localization
J. Cell Sci.,
September 15, 2005;
118(18):
4077 - 4081.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. Prodon, P. Dru, F. Roegiers, and C. Sardet
Polarity of the ascidian egg cortex and relocalization of cER and mRNAs in the early embryo
J. Cell Sci.,
June 1, 2005;
118(11):
2393 - 2404.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Kloc and L. D. Etkin
RNA localization mechanisms in oocytes
J. Cell Sci.,
January 15, 2005;
118(2):
269 - 282.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
E. Chiao, J. Leonard, K. Dickinson, and J. C. Baker
High-throughput functional screen of mouse gastrula cDNA libraries reveals new components of endoderm and mesoderm specification
Genome Res.,
January 1, 2005;
15(1):
44 - 53.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. G. Thomas, L. J. M. Tosar, M. Loschi, J. M. Pasquini, J. Correale, S. Kindler, and G. L. Boccaccio
Staufen Recruitment into Stress Granules Does Not Affect Early mRNA Transport in Oligodendrocytes
Mol. Biol. Cell,
January 1, 2005;
16(1):
405 - 420.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S.-C. Ling, P. S. Fahrner, W. T. Greenough, and V. I. Gelfand
Transport of Drosophila fragile X mental retardation protein-containing ribonucleoprotein granules by kinesin-1 and cytoplasmic dynein
PNAS,
December 14, 2004;
101(50):
17428 - 17433.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. ALLISON, K. CZAPLINSKI, A. GIT, E. ADEGBENRO, F. STENNARD, E. HOULISTON, and N. STANDART
Two distinct Staufen isoforms in Xenopus are vegetally localized during oogenesis
RNA,
November 18, 2004;
10(11):
1751 - 1763.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. Chang, J. Torres, R. A. Lewis, K. L. Mowry, E. Houliston, and M. L. King
Localization of RNAs to the Mitochondrial Cloud in Xenopus Oocytes through Entrapment and Association with Endoplasmic Reticulum
Mol. Biol. Cell,
October 1, 2004;
15(10):
4669 - 4681.
[Abstract]
[Full Text]
[PDF]
|
 |
|
© The Company of Biologists Ltd 2004