spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 3 Nov 2004
doi: 10.1242/dev.01458

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01458v1
131/23/5817    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kemler, R.
Right arrow Articles by Solter, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kemler, R.
Right arrow Articles by Solter, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Stabilization of {beta}-catenin in the mouse zygote leads to premature epithelial-mesenchymal transition in the epiblast


Rolf Kemler*, Andreas Hierholzer, Benoît Kanzler, Stefan Kuppig, Kati Hansen, Makoto M. Taketo, Wilhelmine N. de Vries, Barbara B. Knowles, and Davor Solter
* Author for correspondence (e-mail: kemler{at}immunbio.mpg.de)

Many components of the Wnt/{beta}-catenin signaling pathway are expressed during mouse pre-implantation embryo development, suggesting that this pathway may control cell proliferation and differentiation at this time. We find no evidence for a functional activity of this pathway in cleavage-stage embryos using the Wnt-reporter line, BAT-gal. To further probe the activity of this pathway, we activated {beta}-catenin signaling by mating a zona pellucida3-cre (Zp3-cre) transgenic mouse line with a mouse line containing an exon3-floxed {beta}-catenin allele. The result is expression of a stabilized form of {beta}-catenin, resistant to degradation by the GSK3{beta}-mediated proteasome pathway, expressed in the developing oocyte and in each cell of the resulting embryos. Nuclear localization and signaling function of {beta}-catenin were not observed in cleavage-stage embryos derived from these oocytes. These results indicate that in pre-implantation embryos, molecular mechanisms independent of the GSK3{beta}-mediated ubiquitination and proteasome degradation pathway inhibit the nuclear function of {beta}-catenin. Although the mutant blastocysts initially developed normally, they then exhibited a specific phenotype in the embryonic ectoderm layer of early post-implantation embryos. We show a nuclear function of {beta}-catenin in the mutant epiblast that leads to activation of Wnt/{beta}-catenin target genes. As a consequence, cells of the embryonic ectoderm change their fate, resulting in a premature epithelial-mesenchymal transition.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Fu, M. Jiang, A. J. Mirando, H.-M. I. Yu, and W. Hsu
Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation
PNAS, November 3, 2009; 106(44): 18598 - 18603.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
G. Zheng, J. G. Lyons, T. K. Tan, Y. Wang, T.-T. Hsu, D. Min, L. Succar, G. K. Rangan, M. Hu, B. R. Henderson, et al.
Disruption of E-Cadherin by Matrix Metalloproteinase Directly Mediates Epithelial-Mesenchymal Transition Downstream of Transforming Growth Factor-{beta}1 in Renal Tubular Epithelial Cells
Am. J. Pathol., August 1, 2009; 175(2): 580 - 591.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Grigoryan, P. Wend, A. Klaus, and W. Birchmeier
Deciphering the function of canonical Wnt signals in development and disease: conditional loss- and gain-of-function mutations of {beta}-catenin in mice
Genes & Dev., September 1, 2008; 22(17): 2308 - 2341.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Sumi, N. Tsuneyoshi, N. Nakatsuji, and H. Suemori
Defining early lineage specification of human embryonic stem cells by the orchestrated balance of canonical Wnt/{beta}-catenin, Activin/Nodal and BMP signaling
Development, September 1, 2008; 135(17): 2969 - 2979.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
H. Xie, S. Tranguch, X. Jia, H. Zhang, S. K. Das, S. K. Dey, C. J. Kuo, and H. Wang
Inactivation of nuclear Wnt-{beta}-catenin signaling limits blastocyst competency for implantation
Development, February 15, 2008; 135(4): 717 - 727.
[Abstract] [Full Text] [PDF]

Home page
 Poult. Sci.Home page
W. Wu, R. F. Xu, L. Xiao, H. Xu, and G. Gao
Expression of the -Catenin Gene in the Skin of Embryonic Geese During Feather Bud Development
Poult. Sci., January 1, 2008; 87(1): 204 - 211.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
P. Hohenstein and N. D. Hastie
The many facets of the Wilms' tumour gene, WT1
Hum. Mol. Genet., October 15, 2006; 15(suppl_2): R196 - R201.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Chazaud and J. Rossant
Disruption of early proximodistal patterning and AVE formation in Apc mutants
Development, September 1, 2006; 133(17): 3379 - 3387.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. W. Heiser, J. Lau, M. M. Taketo, P. L. Herrera, and M. Hebrok
Stabilization of {beta}-catenin impacts pancreas growth
Development, May 15, 2006; 133(10): 2023 - 2032.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Z. Den, X. Cheng, M. Merched-Sauvage, and P. J. Koch
Desmocollin 3 is required for pre-implantation development of the mouse embryo
J. Cell Sci., February 1, 2006; 119(3): 482 - 489.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Shi, M. Stahl, L. Lu, and P. Stanley
Canonical Notch Signaling Is Dispensable for Early Cell Fate Specifications in Mammals
Mol. Cell. Biol., November 1, 2005; 25(21): 9503 - 9508.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
P.B. GUPTA, S. MANI, J. YANG, K. HARTWELL, and R.A. WEINBERG
The Evolving Portrait of Cancer Metastasis
Cold Spring Harb Symp Quant Biol, January 1, 2005; 70(0): 291 - 297.
[Abstract] [PDF]


© The Company of Biologists Ltd 2004