spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 16 Jan 2008
doi: 10.1242/dev.015362

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.015362v1
135/4/699    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deng, H.
Right arrow Articles by Johansen, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deng, H.
Right arrow Articles by Johansen, K. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Ectopic histone H3S10 phosphorylation causes chromatin structure remodeling in Drosophila


Huai Deng, Xiaomin Bao, Weili Cai, Melissa J. Blacketer, Andrew S. Belmont, Jack Girton, Jørgen Johansen, and Kristen M. Johansen*
* Author for correspondence (e-mail: kristen{at}iastate.edu)

Histones are subject to numerous post-translational modifications that correlate with the state of higher-order chromatin structure and gene expression. However, it is not clear whether changes in these epigenetic marks are causative regulatory factors in chromatin structure changes or whether they play a mainly reinforcing or maintenance role. In Drosophila phosphorylation of histone H3S10 in euchromatic chromatin regions by the JIL-1 tandem kinase has been implicated in counteracting heterochromatization and gene silencing. Here we show, using a LacI-tethering system, that JIL-1 mediated ectopic histone H3S10 phosphorylation is sufficient to induce a change in higher-order chromatin structure from a condensed heterochromatin-like state to a more open euchromatic state. This effect was absent when a 'kinase dead' LacI-JIL-1 construct without histone H3S10 phosphorylation activity was expressed. Instead, the 'kinase dead' construct had a dominant-negative effect, leading to a disruption of chromatin structure that was associated with a global repression of histone H3S10 phosphorylation levels. These findings provide direct evidence that the epigenetic histone tail modification of H3S10 phosphorylation at interphase can function as a causative regulator of higher-order chromatin structure in Drosophila in vivo.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
K. A. Hines, D. E. Cryderman, K. M. Flannery, H. Yang, M. W. Vitalini, T. Hazelrigg, C. A. Mizzen, and L. L. Wallrath
Domains of Heterochromatin Protein 1 Required for Drosophila melanogaster Heterochromatin Spreading
Genetics, August 1, 2009; 182(4): 967 - 977.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
W. Fischle
Talk is cheap--cross-talk in establishment, maintenance, and readout of chromatin modifications
Genes & Dev., December 15, 2008; 22(24): 3375 - 3382.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Bao, W. Cai, H. Deng, W. Zhang, R. Krencik, J. Girton, J. Johansen, and K. M. Johansen
The COOH-terminal Domain of the JIL-1 Histone H3S10 Kinase Interacts with Histone H3 and Is Required for Correct Targeting to Chromatin
J. Biol. Chem., November 21, 2008; 283(47): 32741 - 32750.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Ciurciu, O. Komonyi, and I. M. Boros
Loss of ATAC-specific acetylation of histone H4 at Lys12 reduces binding of JIL-1 to chromatin and phosphorylation of histone H3 at Ser10
J. Cell Sci., October 15, 2008; 121(20): 3366 - 3372.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
W. Cai, X. Bao, H. Deng, Y. Jin, J. Girton, J. Johansen, and K. M. Johansen
RNA polymerase II-mediated transcription at active loci does not require histone H3S10 phosphorylation in Drosophila
Development, September 1, 2008; 135(17): 2917 - 2925.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Deng, X. Bao, W. Cai, M. J. Blacketer, A. S. Belmont, J. Girton, J. Johansen, and K. M. Johansen
Ectopic histone H3S10 phosphorylation causes chromatin structure remodeling in Drosophila
J. Cell Sci., February 15, 2008; 121(4): e407 - e407.
[Full Text]


© The Company of Biologists Ltd 2008