The fully linked HTML version of this article has now been published.
Development ePress online publication date 12 Jan 2005
doi: 10.1242/dev.01612
Research article
Lsh controls silencing of the imprinted Cdkn1c gene
Tao Fan,
John P. Hagan,
Serguei V. Kozlov,
Colin L. Stewart,
and
Kathrin Muegge*
* Author for correspondence (e-mail: muegge{at}ncifcrf.gov)
Epigenetic regulation, such as DNA methylation plays an important role in the control of imprinting. Lsh, a member of the SNF2 family of chromatin remodeling proteins, controls DNA methylation in mice. To investigate whether Lsh affects imprinting, we examined CpG methylation and allelic expression of individual genes in Lsh-deficient embryos. We report here that loss of Lsh specifically alters expression of the Cdkn1c gene (also known as p57(Kip2)) but does not interfere with maintenance of imprints at the H19, Igf2, Igf2r, Zac1 and Meg9 genes. The reactivation of the silenced paternal Cdkn1c allele correlates closely with a loss of CpG methylation at the 5' DMR at the Cdkn1c promoter, whereas KvDMR1 and DMRs of other imprinted genes were not significantly changed. Chromatin immunoprecipitations demonstrate a direct association of Lsh with the 5' DMR at the Cdkn1c promoter, but not with Kv DMR1 or other imprinted loci. These data suggest that methylation of the 5' DMR plays an important role in the imprinting of the Cdkn1c gene. Furthermore, it suggests that Lsh is not required for maintenance of imprinting marks in general, but is only crucial for imprinting at distinct genomic sites.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
S. K. T. Ooi, A. H. O'Donnell, and T. H. Bestor
Mammalian cytosine methylation at a glance
J. Cell Sci.,
August 15, 2009;
122(16):
2787 - 2791.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Zhou, L. Han, G. Li, and T. Tong
Senescence delay and repression of p16INK4a by Lsh via recruitment of histone deacetylases in human diploid fibroblasts
Nucleic Acids Res.,
August 1, 2009;
37(15):
5183 - 5196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Myant and I. Stancheva
LSH Cooperates with DNA Methyltransferases To Repress Transcription
Mol. Cell. Biol.,
January 1, 2008;
28(1):
215 - 226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Xi, H. Zhu, H. Xu, A. Schmidtmann, T. M. Geiman, and K. Muegge
Lsh controls Hox gene silencing during development
PNAS,
September 4, 2007;
104(36):
14366 - 14371.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C Diatloff-Zito, A Nicole, G Marcelin, H Labit, E Marquis, C Bellanne-Chantelot, and J J Robert
Genetic and epigenetic defects at the 6q24 imprinted locus in a cohort of 13 patients with transient neonatal diabetes: new hypothesis raised by the finding of a unique case with hemizygotic deletion in the critical region
J. Med. Genet.,
January 1, 2007;
44(1):
31 - 37.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Monk, P. Arnaud, S. Apostolidou, F. A. Hills, G. Kelsey, P. Stanier, R. Feil, and G. E. Moore
From the Cover: Limited evolutionary conservation of imprinting in the human placenta
PNAS,
April 25, 2006;
103(17):
6623 - 6628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Takeshima, I. Suetake, H. Shimahara, K. Ura, S.-i. Tate, and S. Tajima
Distinct DNA Methylation Activity of Dnmt3a and Dnmt3b towards Naked and Nucleosomal DNA.
J. Biochem.,
March 1, 2006;
139(3):
503 - 515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Arnaud, K. Hata, M. Kaneda, E. Li, H. Sasaki, R. Feil, and G. Kelsey
Stochastic imprinting in the progeny of Dnmt3L-/- females
Hum. Mol. Genet.,
February 15, 2006;
15(4):
589 - 598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Johnstone, A. J. DuBose, C. R. Futtner, M. D. Elmore, C. I. Brannan, and J. L. Resnick
A human imprinting centre demonstrates conserved acquisition but diverged maintenance of imprinting in a mouse model for Angelman syndrome imprinting defects
Hum. Mol. Genet.,
February 1, 2006;
15(3):
393 - 404.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2005
