spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 9 Feb 2005
doi: 10.1242/dev.01667

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01667v1
132/6/1187    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Montero, J.-A.
Right arrow Articles by Heisenberg, C.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Montero, J.-A.
Right arrow Articles by Heisenberg, C.-P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Shield formation at the onset of zebrafish gastrulation


Juan-Antonio Montero, Lara Carvalho, Michaela Wilsch-Bräuninger, Beate Kilian, Chigdem Mustafa, and Carl-Philipp Heisenberg*
* Author for correspondence (e-mail: heisenberg{at}mpi-cbg.de)

During vertebrate gastrulation, the three germ layers, ectoderm, mesoderm and endoderm are formed, and the resulting progenitor cells are brought into the positions from which they will later contribute more complex tissues and organs. A core element in this process is the internalization of mesodermal and endodermal progenitors at the onset of gastrulation. Although many of the molecules that induce mesendoderm have been identified, much less is known about the cellular mechanisms underlying mesendodermal cell internalization and germ layer formation.

Here we show that at the onset of zebrafish gastrulation, mesendodermal progenitors in dorsal/axial regions of the germ ring internalize by single cell delamination. Once internalized, mesendodermal progenitors upregulate E-Cadherin (Cadherin 1) expression, become increasingly motile and eventually migrate along the overlying epiblast (ectodermal) cell layer towards the animal pole of the gastrula. When E-Cadherin function is compromised, mesendodermal progenitors still internalize, but, with gastrulation proceeding, fail to elongate and efficiently migrate along the epiblast, whereas epiblast cells themselves exhibit reduced radial cell intercalation movements. This indicates that cadherin-mediated cell-cell adhesion is needed within the forming shield for both epiblast cell intercalation, and mesendodermal progenitor cell elongation and migration during zebrafish gastrulation.

Our data provide insight into the cellular mechanisms underlying mesendodermal progenitor cell internalization and subsequent migration during zebrafish gastrulation, and the role of cadherin-mediated cell-cell adhesion in these processes.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
L. Carvalho, J. Stuhmer, J. S. Bois, Y. Kalaidzidis, V. Lecaudey, and C.-P. Heisenberg
Control of convergent yolk syncytial layer nuclear movement in zebrafish
Development, April 15, 2009; 136(8): 1305 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
F. Lin, S. Chen, D. S. Sepich, J. R. Panizzi, S. G. Clendenon, J. A. Marrs, H. E. Hamm, and L. Solnica-Krezel
G{alpha}12/13 regulate epiboly by inhibiting E-cadherin activity and modulating the actin cytoskeleton
J. Cell Biol., March 23, 2009; 184(6): 909 - 921.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
F. Carreira-Barbosa, M. Kajita, V. Morel, H. Wada, H. Okamoto, A. Martinez Arias, Y. Fujita, S. W. Wilson, and M. Tada
Flamingo regulates epiboly and convergence/extension movements through cell cohesive and signalling functions during zebrafish gastrulation
Development, February 1, 2009; 136(3): 383 - 392.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Hammerschmidt and D. Wedlich
Regulated adhesion as a driving force of gastrulation movements
Development, November 15, 2008; 135(22): 3625 - 3641.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H.K. Stelzer
Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy
Science, November 14, 2008; 322(5904): 1065 - 1069.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. Nair and T. F. Schilling
Chemokine Signaling Controls Endodermal Migration During Zebrafish Gastrulation
Science, October 3, 2008; 322(5898): 89 - 92.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Kai, C.-P. Heisenberg, and M. Tada
Sphingosine-1-phosphate receptors regulate individual cell behaviours underlying the directed migration of prechordal plate progenitor cells during zebrafish gastrulation
Development, September 15, 2008; 135(18): 3043 - 3051.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. J. Blanco, A. Barrallo-Gimeno, H. Acloque, A. E. Reyes, M. Tada, M. L. Allende, R. Mayor, and M. A. Nieto
Snail1a and Snail1b cooperate in the anterior migration of the axial mesendoderm in the zebrafish embryo
Development, November 15, 2007; 134(22): 4073 - 4081.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. Ogata, J. Morokuma, T. Hayata, G. Kolle, C. Niehrs, N. Ueno, and K. W.Y. Cho
TGF-beta signaling-mediated morphogenesis: modulation of cell adhesion via cadherin endocytosis
Genes & Dev., July 15, 2007; 21(14): 1817 - 1831.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Babb-Clendenon, Y.-c. Shen, Q. Liu, K. E. Turner, M. S. Mills, G. W. Cook, C. A. Miller, V. H. Gattone II, K. F. Barald, and J. A. Marrs
Cadherin-2 participates in the morphogenesis of the zebrafish inner ear
J. Cell Sci., December 15, 2006; 119(24): 5169 - 5177.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Witzel, V. Zimyanin, F. Carreira-Barbosa, M. Tada, and C.-P. Heisenberg
Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane
J. Cell Biol., December 4, 2006; 175(5): 791 - 802.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. Hong and R. Brewster
N-cadherin is required for the polarized cell behaviors that drive neurulation in the zebrafish
Development, October 1, 2006; 133(19): 3895 - 3905.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
I. Mirkovic and M. Mlodzik
Cooperative activities of Drosophila DE-Cadherin and DN-Cadherin regulate the cell motility process of ommatidial rotation
Development, September 1, 2006; 133(17): 3283 - 3293.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Koppen, B. G. Fernandez, L. Carvalho, A. Jacinto, and C.-P. Heisenberg
Coordinated cell-shape changes control epithelial movement in zebrafish and Drosophila.
Development, July 1, 2006; 133(14): 2671 - 2681.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Link, L. Carvalho, I. Castanon, P. Stockinger, A. Shevchenko, and C.-P. Heisenberg
Identification of regulators of germ layer morphogenesis using proteomics in zebrafish
J. Cell Sci., May 15, 2006; 119(10): 2073 - 2083.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P.-H. Puech, A. Taubenberger, F. Ulrich, M. Krieg, D. J. Muller, and C.-P. Heisenberg
Measuring cell adhesion forces of primary gastrulating cells from zebrafish using atomic force microscopy
J. Cell Sci., September 15, 2005; 118(18): 4199 - 4206.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005