spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 16 Feb 2005
doi: 10.1242/dev.01698

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01698v1
132/6/1283    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyasaka, N.
Right arrow Articles by Yoshihara, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyasaka, N.
Right arrow Articles by Yoshihara, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Robo2 is required for establishment of a precise glomerular map in the zebrafish olfactory system


Nobuhiko Miyasaka, Yuki Sato, Sang-Yeob Yeo, Lara D. Hutson, Chi-Bin Chien, Hitoshi Okamoto, and Yoshihiro Yoshihara*
* Author for correspondence (e-mail: yoshihara{at}brain.riken.go.jp)

Olfactory sensory neurons (OSNs) expressing a given odorant receptor project their axons to specific glomeruli, creating a topographic odor map in the olfactory bulb (OB). The mechanisms underlying axonal pathfinding of OSNs to their precise targets are not fully understood. Here, we demonstrate that Robo2/Slit signaling functions to guide nascent olfactory axons to the OB primordium in zebrafish. robo2 is transiently expressed in the olfactory placode during the initial phase of olfactory axon pathfinding. In the robo2 mutant, astray (ast), early growing olfactory axons misroute ventromedially or posteriorly, and often penetrate into the diencephalon without reaching the OB primordium. Four zebrafish Slit homologs are expressed in regions adjacent to the olfactory axon trajectory, consistent with their role as repulsive ligands for Robo2. Masking of endogenous Slit gradients by ubiquitous misexpression of Slit2 in transgenic fish causes posterior pathfinding errors that resemble the ast phenotype. We also found that the spatial arrangement of glomeruli in OB is perturbed in ast adults, suggesting an essential role for the initial olfactory axon scaffold in determining a topographic glomerular map. These data provide functional evidence for Robo2/Slit signaling in the establishment of olfactory neural circuitry in zebrafish.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Koide, N. Miyasaka, K. Morimoto, K. Asakawa, A. Urasaki, K. Kawakami, and Y. Yoshihara
Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish
PNAS, June 16, 2009; 106(24): 9884 - 9889.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N. Miyasaka, K. Morimoto, T. Tsubokawa, S.-i. Higashijima, H. Okamoto, and Y. Yoshihara
From the Olfactory Bulb to Higher Brain Centers: Genetic Visualization of Secondary Olfactory Pathways in Zebrafish
J. Neurosci., April 15, 2009; 29(15): 4756 - 4767.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. D. Duggan, S. DeMaria, A. Baudhuin, D. Stafford, and J. Ngai
Foxg1 Is Required for Development of the Vertebrate Olfactory System
J. Neurosci., May 14, 2008; 28(20): 5229 - 5239.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. T. Nguyen-Ba-Charvet, T. Di Meglio, C. Fouquet, and A. Chedotal
Robos and Slits Control the Pathfinding and Targeting of Mouse Olfactory Sensory Axons
J. Neurosci., April 16, 2008; 28(16): 4244 - 4249.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. H. Cho, M. Lepine, W. Andrews, J. Parnavelas, and J.-F. Cloutier
Requirement for Slit-1 and Robo-2 in Zonal Segregation of Olfactory Sensory Neuron Axons in the Main Olfactory Bulb
J. Neurosci., August 22, 2007; 27(34): 9094 - 9104.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
N. Miyasaka, H. Knaut, and Y. Yoshihara
Cxcl12/Cxcr4 chemokine signaling is required for placode assembly and sensory axon pathfinding in the zebrafish olfactory system
Development, July 1, 2007; 134(13): 2459 - 2468.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
I. Manzini, C. Brase, T.-W. Chen, and D. Schild
Response profiles to amino acid odorants of olfactory glomeruli in larval Xenopus laevis
J. Physiol., June 1, 2007; 581(2): 567 - 579.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Fouquet, T. Di Meglio, L. Ma, T. Kawasaki, H. Long, T. Hirata, M. Tessier-Lavigne, A. Chedotal, and K. T. Nguyen-Ba-Charvet
Robo1 and Robo2 Control the Development of the Lateral Olfactory Tract
J. Neurosci., March 14, 2007; 27(11): 3037 - 3045.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Sato, N. Miyasaka, and Y. Yoshihara
Hierarchical Regulation of Odorant Receptor Gene Choice and Subsequent Axonal Projection of Olfactory Sensory Neurons in Zebrafish
J. Neurosci., February 14, 2007; 27(7): 1606 - 1615.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Hirata, M. Nakazawa, S.-i. Yoshihara, H. Miyachi, K. Kitamura, Y. Yoshihara, and M. Hibi
Zinc-finger gene Fez in the olfactory sensory neurons regulates development of the olfactory bulb non-cell-autonomously
Development, April 15, 2006; 133(8): 1433 - 1443.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Sato, N. Miyasaka, and Y. Yoshihara
Mutually Exclusive Glomerular Innervation by Two Distinct Types of Olfactory Sensory Neurons Revealed in Transgenic Zebrafish
J. Neurosci., May 18, 2005; 25(20): 4889 - 4897.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005