spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 23 Jun 2005
doi: 10.1242/dev.01916

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01916v1
132/15/3357    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kosinski, M.
Right arrow Articles by Greenstein, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kosinski, M.
Right arrow Articles by Greenstein, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes


Mary Kosinski, Kent McDonald, Joel Schwartz, Ikuko Yamamoto, and David Greenstein*
* Author for correspondence (e-mail: david.greenstein{at}vanderbilt.edu)

The major sperm protein (MSP) is the central cytoskeletal element required for actin-independent motility of nematode spermatozoa. MSP has a dual role in Caenorhabditis elegans reproduction, functioning as a hormone for both oocyte meiotic maturation and ovarian muscle contraction. The identification of the signaling function of MSP raised the question, how do spermatozoa, which are devoid of ribosomes, ER and Golgi, release a cytoplasmic protein lacking a signal sequence? Here, we provide evidence that MSP export occurs by the budding of novel vesicles that have both inner and outer membranes with MSP sandwiched in between. MSP vesicles are apparently labile structures that generate long-range MSP gradients for signaling at the oocyte cell surface. Both spermatozoa and non-motile spermatids bud MSP vesicles, but their stability and signaling properties differ. Budding protrusions from the cell body contain MSP, but not the MSD proteins, which counteract MSP filament assembly. We propose that MSP generates the protrusive force for its own vesicular export.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
J. A. Govindan, S. Nadarajan, S. Kim, T. A. Starich, and D. Greenstein
Somatic cAMP signaling regulates MSP-dependent oocyte growth and meiotic maturation in C. elegans
Development, July 1, 2009; 136(13): 2211 - 2221.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. M. Alcazar, R. Lin, and A. Z. Fire
Transmission Dynamics of Heritable Silencing Induced by Double-Stranded RNA in Caenorhabditis elegans
Genetics, November 1, 2008; 180(3): 1275 - 1288.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Chai, J. Withers, Y. H. Koh, K. Parry, H. Bao, B. Zhang, V. Budnik, and G. Pennetta
hVAPB, the causative gene of a heterogeneous group of motor neuron diseases in humans, is functionally interchangeable with its Drosophila homologue DVAP-33A at the neuromuscular junction
Hum. Mol. Genet., January 15, 2008; 17(2): 266 - 280.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M.-H. Lee, M. Ohmachi, S. Arur, S. Nayak, R. Francis, D. Church, E. Lambie, and T. Schedl
Multiple Functions and Dynamic Activation of MPK-1 Extracellular Signal-Regulated Kinase Signaling in Caenorhabditis elegans Germline Development
Genetics, December 1, 2007; 177(4): 2039 - 2062.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Corrigan, R. Subramanian, and M. A. Miller
Eph and NMDA receptors control Ca2+/calmodulin-dependent protein kinase II activation during C. elegans oocyte meiotic maturation
Development, December 1, 2005; 132(23): 5225 - 5237.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005