spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 15 Feb 2006
doi: 10.1242/dev.02255

This Article
Right arrow Full Text (PDF)
Right arrowOA All Versions of this Article:
dev.02255v1
dev.02255v2
133/6/1133    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jarvis, L. A.
Right arrow Articles by Smith-Bolton, R. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jarvis, L. A.
Right arrow Articles by Smith-Bolton, R. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

(2006)Sprouty proteins are in vivo targets of Corkscrew/SHP-2 tyrosine phosphatases


Lesley A. Jarvis, Stephanie J. Toering, Michael A. Simon, Mark A. Krasnow*, and Rachel K. Smith-Bolton
* Author for correspondence (e-mail: krasnow{at}cmgm.stanford.edu)

Drosophila Corkscrew protein and its vertebrate ortholog SHP-2 (now known as Ptpn11) positively modulate receptor tyrosine kinase (RTK) signaling during development, but how these tyrosine phosphatases promote tyrosine kinase signaling is not well understood. Sprouty proteins are tyrosine-phosphorylated RTK feedback inhibitors, but their regulation and mechanism of action are also poorly understood. Here, we show that Corkscrew/SHP-2 proteins control Sprouty phosphorylation and function. Genetic experiments demonstrate that Corkscrew/SHP-2 and Sprouty proteins have opposite effects on RTK-mediated developmental events in Drosophila and an RTK signaling process in cultured mammalian cells, and the genes display dose-sensitive genetic interactions. In cultured cells, inactivation of SHP-2 increases phosphorylation on the critical tyrosine of Sprouty 1. SHP-2 associates in a complex with Sprouty 1 in cultured cells and in vitro, and a purified SHP-2 protein dephosphorylates the critical tyrosine of Sprouty 1. Substrate-trapping forms of Corkscrew bind Sprouty in cultured Drosophila cells and the developing eye. These results identify Sprouty proteins as in vivo targets of Corkscrew/SHP-2 tyrosine phosphatases and show how Corkscrew/SHP-2 proteins can promote RTK signaling by inactivating a feedback inhibitor. We propose that this double-negative feedback circuit shapes the output profile of RTK signaling events.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J EndocrinolHome page
G R Guy, R A Jackson, P Yusoff, and S Y Chow
Sprouty proteins: modified modulators, matchmakers or missing links?
J. Endocrinol., November 1, 2009; 203(2): 191 - 202.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
F. Edwin, K. Anderson, C. Ying, and T. B. Patel
Intermolecular Interactions of Sprouty Proteins and Their Implications in Development and Disease
Mol. Pharmacol., October 1, 2009; 76(4): 679 - 691.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. S. Zhang, E. Hao, J. Yu, W. Liu, J. Wang, F. Levine, and G.-S. Feng
Coordinated regulation by Shp2 tyrosine phosphatase of signaling events controlling insulin biosynthesis in pancreatic {beta}-cells
PNAS, May 5, 2009; 106(18): 7531 - 7536.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. Horowitz and M. Simons
Branching Morphogenesis
Circ. Res., October 10, 2008; 103(8): 784 - 795.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. G. Langdon, S. C. Goetz, A. E. Berg, J. T. Swanik, and F. L. Conlon
SHP-2 is required for the maintenance of cardiac progenitors
Development, November 15, 2007; 134(22): 4119 - 4130.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Bundschu, U. Walter, and K. Schuh
The VASP-Spred-Sprouty Domain Puzzle
J. Biol. Chem., December 1, 2006; 281(48): 36477 - 36481.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006