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Development ePress online publication date 22 Mar 2006
doi: 10.1242/dev.02336
Research article
Integrin
6
1-laminin interactions regulate early myotome formation in the mouse embryo
Fernanda Bajanca,
Marta Luz,
Karine Raymond,
Gabriel G. Martins,
Arnoud Sonnenberg,
Shahragim Tajbakhsh,
Margaret Buckingham,
and
Sólveig Thorsteinsdóttir*
* Author for correspondence (e-mail: solveig{at}fc.ul.pt)
We addressed the potential role of cell-laminin interactions during epaxial myotome formation in the mouse embryo. Assembly of the myotomal laminin matrix occurs as epaxial myogenic precursor cells enter the myotome. Most Myf5-positive and myogenin-negative myogenic precursor cells localise near assembled laminin, while myogenin-expressing cells are located either away from this matrix or in areas where it is being assembled. In Myf5nlacZ/nlacZ (Myf5-null) embryos, laminin, collagen type IV and perlecan are present extracellularly near myogenic precursor cells, but do not form a basement membrane and cells are not contained in the myotomal compartment. Unlike wild-type myogenic precursor cells, Myf5-null cells do not express the
6
1 integrin, a laminin receptor, suggesting that integrin
6
1-laminin interactions are required for myotomal laminin matrix assembly. Blocking
6
1-laminin binding in cultured wild-type mouse embryo explants resulted in dispersion of Myf5-positive cells, a phenotype also seen in Myf5nlacZ/nlacZ embryos. Furthermore, inhibition of
6
1 resulted in an increase in Myf5 protein and ectopic myogenin expression in dermomyotomal cells, suggesting that
6
1-laminin interactions normally repress myogenesis in the dermomyotome. We conclude that Myf5 is required for maintaining
6
1 expression on myogenic precursor cells, and that
6
1 is necessary for myotomal laminin matrix assembly and cell guidance into the myotome. Engagement of laminin by
6
1 also plays a role in maintaining the undifferentiated state of cells in the dermomyotome prior to their entry into the myotome.

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© The Company of Biologists Ltd 2006