spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 3 Aug 2006
doi: 10.1242/dev.02506

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.02506v1
133/17/3451    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Francis, R. J.B.
Right arrow Articles by Lo, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Francis, R. J.B.
Right arrow Articles by Lo, C. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Primordial germ cell deficiency in the connexin 43 knockout mouse arises from apoptosis associated with abnormal p53 activation


Richard J.B. Francis and Cecilia W. Lo*
* Author for correspondence (e-mail: loc{at}nhlbi.nih.gov)

Connexin 43 knockout (Cx43{alpha}1KO) mice exhibit germ cell deficiency, but the underlying cause for the germ cell defect was unknown. Using an Oct4-GFP reporter transgene, we tracked the distribution and migration of primordial germ cells (PGCs) in the Cx43{alpha}1KO mouse embryo. Analysis with dye injections showed PGCs are gap-junction-communication competent, with dye coupling being markedly reduced in Cx43{alpha}1-deficient PGCs. Time-lapse videomicroscopy and motion analysis showed that the directionality and speed of cell motility were reduced in the Cx43{alpha}1KO PGCs. This was observed both in E8.5 and E11.5 embryos. By contrast, PGC abundance did not differ between wild-type and heterozygous/homozygous Cx43{alpha}1KO embryos until E11.5, when a marked reduction in PGC abundance was detected in the homozygous Cx43{alpha}1KO embryos. This was accompanied by increased PGC apoptosis and increased expression of activated p53. Injection of {alpha}-pifithrin, a p53 antagonist, inhibited PGC apoptosis and prevented the loss of PGC. Analysis using a cell adhesion assay indicated a reduction in {beta}1-integrin function in the Cx43{alpha}1KO PGCs. Together with the abnormal activation of p53, these findings suggest the possibility of anoikis-mediated apoptosis. Overall, these findings show Cx43{alpha}1 is essential for PGC survival, with abnormal p53 activation playing a crucial role in the apoptotic loss of PGCs in the Cx43{alpha}1KO mouse embryos.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
G. La Sala, D. Farini, and M. De Felici
Proapoptotic Effects of Lindane on Mouse Primordial Germ Cells
Toxicol. Sci., April 1, 2009; 108(2): 445 - 451.
[Abstract] [Full Text] [PDF]

Home page
 Evid Based Complement Alternat MedHome page
C. Shang
Prospective Tests on Biological Models of Acupuncture
Evid. Based Complement. Altern. Med., March 1, 2009; 6(1): 31 - 39.
[Abstract] [Full Text] [PDF]

Home page
 DMMHome page
D. Tong, D. Colley, R. Thoo, T. Y. Li, I. Plante, D. W. Laird, D. Bai, and G. M. Kidder
Oogenesis defects in a mutant mouse model of oculodentodigital dysplasia
Dis. Model. Mech., March 1, 2009; 2(3-4): 157 - 167.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. D. Pooley, K. L. Moynihan, V. Soukoulis, S. Reddy, R. Francis, C. Lo, L.-J. Ma, and D. M. Bader
Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport
J. Cell Sci., October 15, 2008; 121(20): 3413 - 3421.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. Yamada, K. D. Davis, and C. R. Coffman
Programmed cell death of primordial germ cells in Drosophila is regulated by p53 and the Outsiders monocarboxylate transporter
Development, January 15, 2008; 135(2): 207 - 216.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. Brehm, M. Zeiler, C. Ruttinger, K. Herde, M. Kibschull, E. Winterhager, K. Willecke, F. Guillou, C. Lecureuil, K. Steger, et al.
A Sertoli Cell-Specific Knockout of Connexin43 Prevents Initiation of Spermatogenesis
Am. J. Pathol., July 1, 2007; 171(1): 19 - 31.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006