The fully linked HTML version of this article has now been published.
Development ePress online publication date 16 Aug 2006
doi: 10.1242/dev.02521
Research article
Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3
Gang Wu
and
R. Scott Poethig*
* Author for correspondence (e-mail: spoethig{at}sas.upenn.edu)
SPL3, SPL4 and SPL5 (SPL3/4/5) are closely related members of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE family of transcription factors in Arabidopsis, and have a target site for the microRNA miR156 in their 3' UTR. The phenotype of Arabidopsis plants constitutively expressing miR156-sensitive and miR156-insensitive forms of SPL3/4/5 revealed that all three genes promote vegetative phase change and flowering, and are strongly repressed by miR156. Constitutive expression of miR156a prolonged the expression of juvenile vegetative traits and delayed flowering. This phenotype was largely corrected by constitutive expression of a miR156-insensitive form of SPL3. The juvenile-to-adult transition is accompanied by a decrease in the level of miR156 and an increase in the abundance of SPL3 mRNA. The complementary effect of hasty on the miR156 and SPL3 transcripts, as well as the miR156-dependent temporal expression pattern of a 35S::GUS-SPL3 transgene, suggest that the decrease in miR156 is responsible for the increase in SPL3 expression during this transition. SPL3 mRNA is elevated by mutations in ZIPPY/AGO7, RNA DEPENDENT RNA POLYMERASE 6 (RDR6) and SUPPRESSOR OF GENE SILENCING 3 (SGS3), indicating that it is directly or indirectly regulated by RNAi. However, our results indicate that RNAi does not contribute to the temporal expression pattern of this gene. We conclude that vegetative phase change in Arabidopsis is regulated by an increase in the expression of SPL3 and probably also SPL4 and SPL5, and that this increase is a consequence of a decrease in the level of miR156.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Baga, D. B. Fowler, and R. N. Chibbar
Identification of genomic regions determining the phenological development leading to floral transition in wheat (Triticum aestivum L.)
J. Exp. Bot.,
August 1, 2009;
60(12):
3575 - 3585.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Mitsuda and M. Ohme-Takagi
Functional Analysis of Transcription Factors in Arabidopsis
Plant Cell Physiol.,
July 1, 2009;
50(7):
1232 - 1248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. D. Pant, M. Musialak-Lange, P. Nuc, P. May, A. Buhtz, J. Kehr, D. Walther, and W.-R. Scheible
Identification of Nutrient-Responsive Arabidopsis and Rapeseed MicroRNAs by Comprehensive Real-Time Polymerase Chain Reaction Profiling and Small RNA Sequencing
Plant Physiology,
July 1, 2009;
150(3):
1541 - 1555.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Smith, M. R. Willmann, G. Wu, T. Z. Berardini, B. Moller, D. Weijers, and R. S. Poethig
Cyclophilin 40 is required for microRNA activity in Arabidopsis
PNAS,
March 31, 2009;
106(13):
5424 - 5429.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Usami, G. Horiguchi, S. Yano, and H. Tsukaya
The more and smaller cells mutants of Arabidopsis thaliana identify novel roles for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes in the control of heteroblasty
Development,
March 15, 2009;
136(6):
955 - 964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. Kim, H. R. Woo, J. Kim, P. O. Lim, I. C. Lee, S. H. Choi, D. Hwang, and H. G. Nam
Trifurcate Feed-Forward Regulation of Age-Dependent Cell Death Involving miR164 in Arabidopsis
Science,
February 20, 2009;
323(5917):
1053 - 1057.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-G. Joung and Z. Fei
Identification of microRNA regulatory modules in Arabidopsis via a probabilistic graphical model
Bioinformatics,
February 1, 2009;
25(3):
387 - 393.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Coupland
Mechanisms and variation in plant development: sorting the wood from the trees in Vermont
Development,
December 1, 2008;
135(23):
3813 - 3816.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Lauter, M. J. Moscou, J. Habiger, and S. P. Moose
Quantitative Genetic Dissection of Shoot Architecture Traits in Maize: Towards a Functional Genomics Approach
The Plant Genome,
November 1, 2008;
1(2):
99 - 110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-I. Itoh, Y. Sato, and Y. Nagato
The SHOOT ORGANIZATION2 Gene Coordinates Leaf Domain Development Along the Central-Marginal Axis in Rice
Plant Cell Physiol.,
August 1, 2008;
49(8):
1226 - 1236.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-W. Wang, R. Schwab, B. Czech, E. Mica, and D. Weigel
Dual Effects of miR156-Targeted SPL Genes and CYP78A5/KLUH on Plastochron Length and Organ Size in Arabidopsis thaliana
PLANT CELL,
May 1, 2008;
20(5):
1231 - 1243.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. M. Ehrenreich and M. D. Purugganan
Sequence Variation of MicroRNAs and Their Binding Sites in Arabidopsis
Plant Physiology,
April 1, 2008;
146(4):
1974 - 1982.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. George, S. M. Romanowsky, J. F. Harper, and R. A. Sharrock
The ACA10 Ca2+-ATPase Regulates Adult Vegetative Development and Inflorescence Architecture in Arabidopsis
Plant Physiology,
February 1, 2008;
146(2):
716 - 728.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Flowering Newsletter bibliography for 2006
J. Exp. Bot.,
April 20, 2007;
(2007)
erm028v2.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R.S. POETHIG, A. PERAGINE, M. YOSHIKAWA, C. HUNTER, M. WILLMANN, and G. WU
The Function of RNAi in Plant Development
Cold Spring Harb Symp Quant Biol,
January 1, 2006;
71(0):
165 - 170.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G.J. HANNON, F.V. RIVAS, E.P. MURCHISON, and J.A. STEITZ
The Expanding Universe of Noncoding RNAs
Cold Spring Harb Symp Quant Biol,
January 1, 2006;
71(0):
551 - 564.
[Abstract]
[PDF]
|
|
|
© The Company of Biologists Ltd 2006
