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Development ePress online publication date 13 Sep 2006
doi: 10.1242/dev.02583

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Research article

Semaphorin 3d promotes cell proliferation and neural crest cell development downstream of TCF in the zebrafish hindbrain


Jason D. Berndt and Mary C. Halloran*
* Author for correspondence (e-mail: mchalloran{at}wisc.edu)

Neural crest cells (NCCs) are pluripotent migratory cells that are crucial to the development of the peripheral nervous system, pigment cells and craniofacial cartilage and bone. NCCs are specified within the dorsal ectoderm and undergo an epithelial to mesenchymal transition (EMT) in order to migrate to target destinations where they differentiate. Here we report a role for a member of the semaphorin family of cell guidance molecules in NCC development. Morpholino-mediated knockdown of Sema3d inhibits the proliferation of hindbrain neuroepithelial cells. In addition, Sema3d knockdown reduces markers of migratory NCCs and disrupts NCC-derived tissues. Similarly, expression of a dominant-repressor form of TCF ({Delta}TCF) reduces hindbrain cell proliferation and leads to a disruption of migratory NCC markers. Moreover, expression of {Delta}TCF downregulates sema3d RNA expression. Finally, Sema3d overexpression rescues reduced proliferation caused by {Delta}TCF expression, suggesting that Sema3d lies downstream of Wnt/TCF signaling in the molecular pathway thought to control cell cycle in NCC precursors.




© The Company of Biologists Ltd 2006