The fully linked HTML version of this article has now been published.
Development ePress online publication date 14 Feb 2007
doi: 10.1242/dev.02781
Research article
Ptf1a is essential for the differentiation of GABAergic and glycinergic amacrine cells and horizontal cells in the mouse retina
Hassan Nakhai*,
Saadettin Sel,
Jack Favor,
Lidia Mendoza-Torres,
Friedrich Paulsen,
Gernot I.W. Duncker,
and
Roland M. Schmid
* Author for correspondence (e-mail: hassan.nakhai{at}lrz.tum.de)
Basic helix-loop-helix (bHLH) transcription factors are important regulators of retinal neurogenesis. In the developing retina, proneural bHLH genes have highly defined expressions, which are influenced by pattern formation and cell-specification pathways. We report here that the tissue-specific bHLH transcription factor Ptf1a (also known as PTF1-p48) is expressed from embryonic day 12.5 of gestation (E12.5) to postnatal day 3 (P3) during retinogenesis in the mouse. Using recombination-based lineage tracing, we provide evidence that Ptf1a is expressed in precursors of amacrine and horizontal cells. Inactivation of Ptf1a in the developing retina led to differentiation arrest of amacrine and horizontal precursor cells in addition to partial transdifferentiation of Ptf1a-expressing precursor cells to ganglion cells. Analysis of late cell-type-specific markers revealed the presence of a small population of differentiated amacrine cells, whereas GABAergic and glycinergic amacrine cells, as well as horizontal cells, were completely missing in Ptf1a-knockout retinal explants. We conclude that Ptf1a contributes to the differentiation of horizontal cells and types of amacrine cells during mouse retinogenesis.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
D. M. Meredith, T. Masui, G. H. Swift, R. J. MacDonald, and J. E. Johnson
Multiple Transcriptional Mechanisms Control Ptf1a Levels during Neural Development Including Autoregulation by the PTF1-J Complex
J. Neurosci.,
September 9, 2009;
29(36):
11139 - 11148.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. Henke, T. K. Savage, D. M. Meredith, S. M. Glasgow, K. Hori, J. Dumas, R. J. MacDonald, and J. E. Johnson
Neurog2 is a direct downstream target of the Ptf1a-Rbpj transcription complex in dorsal spinal cord
Development,
September 1, 2009;
136(17):
2945 - 2954.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Jiang and M. Xiang
Subtype Specification of GABAergic Amacrine Cells by the Orphan Nuclear Receptor Nr4a2/Nurr1
J. Neurosci.,
August 19, 2009;
29(33):
10449 - 10459.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Fujiyama, M. Yamada, M. Terao, T. Terashima, H. Hioki, Y. U. Inoue, T. Inoue, N. Masuyama, K. Obata, Y. Yanagawa, et al.
Inhibitory and excitatory subtypes of cochlear nucleus neurons are defined by distinct bHLH transcription factors, Ptf1a and Atoh1
Development,
June 15, 2009;
136(12):
2049 - 2058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Khasawneh, M. D. Schulz, A. Walch, J. Rozman, M. H. de Angelis, M. Klingenspor, A. Buck, M. Schwaiger, D. Saur, R. M. Schmid, et al.
Inflammation and mitochondrial fatty acid {beta}-oxidation link obesity to early tumor promotion
PNAS,
March 3, 2009;
106(9):
3354 - 3359.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. F. Medina, C. Mazerolle, Y. Wang, N. G. Berube, S. Coupland, R. J. Gibbons, V. A. Wallace, and D. J. Picketts
Altered visual function and interneuron survival in Atrx knockout mice: inference for the human syndrome
Hum. Mol. Genet.,
March 1, 2009;
18(5):
966 - 977.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Fuhrmann, A. N. Riesenberg, A. M. Mathiesen, E. C. Brown, M. L. Vetter, and N. L. Brown
Characterization of a Transient TCF/LEF-Responsive Progenitor Population in the Embryonic Mouse Retina
Invest. Ophthalmol. Vis. Sci.,
January 1, 2009;
50(1):
432 - 440.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Nakhai, J. T. Siveke, L. Mendoza-Torres, and R. M. Schmid
Conditional inactivation of Myc impairs development of the exocrine pancreas
Development,
October 1, 2008;
135(19):
3191 - 3196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Nakhai, J. T. Siveke, B. Klein, L. Mendoza-Torres, P. K. Mazur, H. Algul, F. Radtke, L. Strobl, U. Zimber-Strobl, and R. M. Schmid
Conditional ablation of Notch signaling in pancreatic development
Development,
August 15, 2008;
135(16):
2757 - 2765.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Seidler, A. Schmidt, U. Mayr, H. Nakhai, R. M. Schmid, G. Schneider, and D. Saur
A Cre-loxP-based mouse model for conditional somatic gene expression and knockdown in vivo by using avian retroviral vectors
PNAS,
July 22, 2008;
105(29):
10137 - 10142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. S. Dong, E. Provost, S. D. Leach, and D. Y.R. Stainier
Graded levels of Ptf1a differentially regulate endocrine and exocrine fates in the developing pancreas
Genes & Dev.,
June 1, 2008;
22(11):
1445 - 1450.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hori, J. Cholewa-Waclaw, Y. Nakada, S. M. Glasgow, T. Masui, R. M. Henke, H. Wildner, B. Martarelli, T. M. Beres, J. A. Epstein, et al.
A nonclassical bHLH Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling
Genes & Dev.,
January 15, 2008;
22(2):
166 - 178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Yamada, M. Terao, T. Terashima, T. Fujiyama, Y. Kawaguchi, Y.-i. Nabeshima, and M. Hoshino
Origin of Climbing Fiber Neurons and Their Developmental Dependence on Ptf1a
J. Neurosci.,
October 10, 2007;
27(41):
10924 - 10934.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2007
