spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 7 Feb 2007
doi: 10.1242/dev.02806


This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.02806v1
134/6/1111    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, Y.
Right arrow Articles by Drummond, I. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, Y.
Right arrow Articles by Drummond, I. A.

Research article

Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros


Yan Liu, Narendra Pathak, Albrecht Kramer-Zucker, and Iain A. Drummond*
* Author for correspondence (e-mail: idrummon{at}receptor.mgh.harvard.edu)

Epithelial tubules consist of multiple cell types that are specialized for specific aspects of organ function. In the zebrafish pronephros, multiciliated cells (MCCs) are specialized for fluid propulsion, whereas transporting epithelial cells recover filtered-blood solutes. These cell types are distributed in a 'salt-and-pepper' fashion in the pronephros, suggesting that a lateral inhibition mechanism may play a role in their differentiation. We find that the Notch ligand Jagged 2 is expressed in MCCs and that notch3 is expressed in pronephric epithelial cells. Morpholino knockdown of either jagged 2 or notch3, or mutation in mind bomb (in which Notch signaling is impaired), dramatically expands ciliogenic gene expression, whereas ion transporter expression is lost, indicating that pronephric cells are transfated to MCCs. Conversely, ectopic expression of the Notch1a intracellular domain represses MCC differentiation. Gamma-secretase inhibition using DAPT demonstrated a requirement for Notch signaling early in pronephric development, before the pattern of MCC differentiation is apparent. Strikingly, we find that jagged 2 knockdown generates extra cilia and is sufficient to rescue the kidney cilia mutant double bubble. Our results indicate that Jagged 2/Notch signaling modulates the number of multiciliated versus transporting epithelial cells in the pronephros by way of a genetic pathway involving repression of rfx2, a key transcriptional regulator of the ciliogenesis program.




This article has been cited by other articles:


Home page
DevelopmentHome page
S. P. Mudumana, D. Hentschel, Y. Liu, A. Vasilyev, and I. A. Drummond
odd skipped related1 reveals a novel role for endoderm in regulating kidney versus vascular cell fate
Development, October 15, 2008; 135(20): 3355 - 3367.
[Abstract] [Full Text] [PDF]


Home page
Physiol. GenomicsHome page
D. Markovich, A. Romano, C. Storelli, and T. Verri
Functional and structural characterization of the zebrafish Na+-sulfate cotransporter 1 (NaS1) cDNA and gene (slc13a1)
Physiol Genomics, August 1, 2008; 34(3): 256 - 264.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
N. Pathak, T. Obara, S. Mangos, Y. Liu, and I. A. Drummond
The Zebrafish fleer Gene Encodes an Essential Regulator of Cilia Tubulin Polyglutamylation
Mol. Biol. Cell, November 1, 2007; 18(11): 4353 - 4364.
[Abstract] [Full Text] [PDF]


Home page
J. Am. Soc. Nephrol.Home page
R. Kopan, H.-T. Cheng, and K. Surendran
Molecular Insights into Segmentation along the Proximal Distal Axis of the Nephron
J. Am. Soc. Nephrol., July 1, 2007; 18(7): 2014 - 2020.
[Abstract] [Full Text] [PDF]




© The Company of Biologists Ltd 2007