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Neural crest progenitors of the melanocyte lineage: coat colour patterns revisited

Alison L. Wilkie, Siobhán A. Jordan and Ian J. Jackson

MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, UK



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  		Fig. 1. Labelled melanoblasts in Dct-lacZ control embryos of different ages. (A) E11.5. (B) E12.5. (C) E13.5. (D) E14.5. (E) E15.5. (F) E16.5. (A,B) Labelled melanoblasts can be seen at high density flanking the neural tube and in the cervical region. (C) By E13.5, melanoblasts have begun to disperse ventrally, and a significant proportion of the body and face have been populated. (D) By E14.5, melanoblasts are more numerous and almost the entire embryo has been colonised, including the ventrum and crown of the head. (E,F) Melanoblasts continue to increase in number and begin to colonise hair follicles (E15.5-E16.5). Scale bars: 0.8 mm in B; 1.6 mm in D; 3.2 mm in F, respectively.

 


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  		Fig. 2. Examples of Dct-lacZ {leftrightarrow} non-transgenic chimaeric embryos. (Ai) Ventral view of an E12.5 chimaera with labelled melanoblasts in the face and tail. (Aii-Aiii) Right and left side views of the same embryo. (B) E12.5 chimaera showing a dilution of labelled melanoblasts between the limbs (marked by arrows). (Ci-Cii) Left and right sides of an E13.5 chimaera showing distinctive gaps (marked by arrows) lacking labelled melanoblasts between the limbs. (D) Head and face of an E15.5 chimaera showing labelled melanoblasts in the snout, pinna and around the eye. (E) E16.5 chimaera showing discrete patches of labelled melanoblasts.

 


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  		Fig. 3. Examples of melanoblast clones generated in preliminary screening of Dct-laacZ transgenic lines. (A) Large E13.5 face clone in line Tg(Dct-laacZ)195Jkn. (B) Small E13.5 face clone in line Tg(Dct-laacZ)195Jkn. (C) E13.5 clone at the forelimb level in line Tg(Dct-laacZ)202Jkn.

 


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  		Fig. 4. Transgenes are expressed in equivalent domains in control Tg(Dct-lacZ) and Tg(Dct-laacZ)195Jkn embryos. Whole-mount in situ hybridisation using a lacZ riboprobe. (A,D) E11.5 Tg(Dct-lacZ) embryo. (B,E) E11.5 Tg(Dct-laacZ)195Jkn embryo. (C,F) E11.5 non-transgenic embryo. Scale bars: 400 µm.

 


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  		Fig. 5. Examples of melanoblast clones in Tg(Dct-laacZ)195Jkn mosaic embryos. (A-C) Face clones. (A) E11.5. (B) E13.0. (C) E13.5. (D-F) Clones in the cervical region. (D) E11.5. (E) E12.5. (F) E13.5. (G,H) Trunk clones. (Gi,Gii) E12.5 (left and right hand sides of the same embryo). (H) E14.5. See text for details and discussion.

 




© The Company of Biologists Ltd 2002