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Fig. 2. The Kinesin heavy chain is required for ooplasmic movement at stage 9.
(A-D) Movement of GFP-Staufen particles in wild-type (A,B) and Khc
mutant (C,D) egg chambers. Each image shows three consecutive time points of a
time lapse movie that have been imported into the red, green and blue channels
of a Photoshop file. In the nurse cell cytoplasm, the movement of GFP-Staufen
particles in the Khc mutant is indistinguishable from that in wild
type. In the ooplasm, however, most of the GFP-Staufen particles are static in
the Khc mutants (white particles), and the ones that do move lie
close to the ring canals. (E-J) Movement of particles that reflect 568 nm
light in wild-type (E,F), Khc (G,H) and mago nashi (I,J)
mutant egg chambers. In the Khc mutant, most of the particles in the
oocyte are stationery (white particles), whereas all the particles in wild
type and in mago nashi mutant oocytes move between time points. In
staufen, barentsz and Tropomyosin II mutant oocytes, these
particles also show the same movement as in wild type (I. M. P. and D. St J.,
unpublished). (K-P) Movement of particles that reflect 568 nm light in wild
type (K-M) and Khc mutant (N-P) egg chambers. The oocytes were
scanned either once (1; K,N) or in a continuous manner using the Kalman
function of the confocal microscope for 15 (15; L, O) or 30 (30; M, P) scans,
and the composite image is shown. Each scan lasts 7 seconds, so the images
represent either 7 seconds (K,N), 105 seconds (L,O) or 210 seconds (M,P) of
real time. The ooplasmic streaming observed in a stage 9 wild-type oocyte
(K-M) is completely abolished in the Khc mutant oocyte (N-P). (Q,R)
Quantitation of the movement of GFP-Staufen particles in wild-type and
Khc mutant egg chambers. Movement of GFP-Staufen particles in
wild-type (black boxes) and Khc mutant (grey boxes) egg chambers. The
graphic shows the number of particles (x-axis) that move with a
certain speed (y-axis) in both the nurse cells (Q) and the oocyte
(R). In Khc mutant egg chambers, no movement of GFP-Staufen particles
within the oocyte was observed, although the motion of these particles within
the nurse cells was unaffected. This analysis does not consider the movements
of the particles in the z-axis, but a more detailed analysis of the
time lapse movies reveals that the particles move much less in this
dimension.
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