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Fig. 1. Correlation of Wnt5a and Wnt11 expression with myogenic
differentiation. The expression of Wnt5a (A-C,J-M), MyoD
(D-F,N) and Wnt11 (G-I,N) in stage 22 (A,D,G), 25 (B,E,H) and 27
(C,F,I) wing buds was determined by whole-mount in situ hybridization. (J-N)
Transverse vibratome sections of wing buds expressing Wnt5a (J-M) and
Wnt11 and MyoD (N) at stages 22 (J,K), 26 (N) and 27 (L,M).
In (A-M), expression is shown in purple/red. In (N), MyoD expression
is shown in red and Wnt11 expression is shown in purple. In (A-N),
anterior is uppermost and, in (A-I), distal is to the right. In the vibratome
sections, (J) is more distal than (K), and (L) is more distal than (M). In
(M), developing cartilage is circled in red, whereas developing muscle is
circled in black. DM, dorsal muscle mass; R, radius; U, ulna; VM, ventral
muscle mass. Scale bars: 100 µm in A,D,G; 150 µm in B,C,E,F,H,I; 125
µm in J,K; 150 µm in L,M.
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Wnt11 (F,H) at stage 18-20, and
the contralateral control wings (A,C,E,G), which have been visualized with
antibody A4.1025, which recognizes slow and fast MyHCs (A,B,E,F, green), and
antibody A4.840, which recognizes slow MyHC (C,D,G,H, red). (A-D) Sections
through the whole wing. (E-H) High power pictures of the EMU and EDC muscles.
The arrows in (A,B,G,H) compare equivalent muscles, showing the changes in
muscle development. In (B), the muscles are absent (EMU, SUP) or are decreased
in size (EMR, FCU). In H, the EMU muscle has more slow fibres. EDC, extensor
digitorum communis; EIL, extensor indicis longus; EMR, extensor metacarpi
radialis; EMU, extensor metacarpi ulnaris; FDP, flexor digitorum profundus;
SUP, supinator. Scale bars: 200 µm in AD; 100 µm in E-L.
